Project description:Two Zygnematophyceaen algae (sister-clade of the Embryophytes) were investigated: Mesotaenium endlicherianum SAG12.97 and Zygnema circumcarinatum SAG698-1b. The algae were grown on agar plates and submitted to osmotic stress for 6 or 25 hours by transferring them to a plates supplemented with either 0.8M mannitol or 0.15M NaCl. We investigated their osmotic response with the use of transcriptomics, proteomics, metabolomics, photophysiology analysis, sugar analysis, microscopy, and immunocytochemical glycoprotein analysis.
2025-08-12 | PXD060327 | Pride
Project description:Stress profiling of Physcomitrium patens and Zygnema circumcarinatum
Project description:This model was reconstructed from single-nucleus RNA-seq (snRNA-seq) data of human postmortem brain and curated using published metabolomics data from human iPSC-derived neurons and cerebrospinal fluid (CSF), together with gene expression data from the Human Protein Atlas. It more accurately simulates human neuronal metabolic flux in neurodegenerative conditions such as Alzheimer's disease (AD).