Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Extracellular matrix remodelling is associated with muscle force increase in overloaded mouse plantaris muscle.

ABSTRACT: Extracellular matrix remodelling is associated with muscle force increase in overloaded mouse plantaris muscle.

PROVIDER: PRJNA524535 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Extracellular matrix remodelling is associated with muscle force increase in overloaded mouse plantaris muscle.

Project description:Aims: Transforming growth factor-β (TGF-β) signalling is thought to contribute to the remodelling of extracellular matrix (ECM) of skeletal muscle and to functional decline in patients with muscular dystrophies. We wanted to determine the role of TGF-β-induced ECM remodelling in dystrophic muscle. Methods: We experimentally induced the pathological hallmarks of severe muscular dystrophy by mechanically overloading the plantaris muscle in mice. Furthermore, we determined the role of TGF-β signalling on dystrophic tissue modulation and on muscle function by (i) overloading myostatin knockout (Mstn-/- ) mice and (ii) by additional pharmacological TGF-β inhibition via halofuginone. Results: Transcriptome analysis of overloaded muscles revealed upregulation predominantly of genes associated with ECM, inflammation and metalloproteinase activity. Histology revealed in wild-type mice signs of severe muscular dystrophy including myofibres with large variation in size and internalized myonuclei, as well as increased ECM deposition. At the same time, muscle weight had increased by 208% and muscle force by 234%. Myostatin deficiency blunted the effect of overload on muscle mass (59% increase) and force (76% increase), while having no effect on ECM deposition. Concomitant treatment with halofuginone blunted overload-induced muscle hypertrophy and muscle force increase, while reducing ECM deposition and increasing myofibre size. Conclusions: ECM remodelling is associated with an increase in muscle mass and force in overload-modelled dystrophic muscle. Lack of myostatin is not advantageous and inhibition of ECM deposition by halofuginone is disadvantageous for muscle plasticity in response to stimuli that induce dystrophic muscle.

2020-08-01 | GSE127255 | GEO

Influence of low and high force muscle activity on paralyzed muscle gene expression

Project description:We used a novel approach to study the acute effect of two frequencies of stimulation (20 Hz and 5 Hz; high and low force, respectively) on gene regulation in people with chronic paralysis. Three hours after the completion of the electrical stimulation protocol (5 Hz or 20 Hz), we sampled the vastus lateralis muscle and examined genes involved with metabolic transcription, glycolysis, oxidative phosphorylation, and mitochondria remodelling. We discovered that the 5 Hz stimulation, despite generating a lower overall force, induced a 5-6 fold increase (p<0.05) in key metabolic transcription factors including PGC-1?, NR4A3, and ABRA. Neither protocol showed a robust regulation of genes for glycolysis, oxidative phosphorylation, and mitochondria remodelling. We analyzed skeletal muscle using the Affymetrix Human Exon 1.0 ST platform. Array data was processed by Partek Genomic Suites.

2015-08-01 | E-GEOD-64683 | biostudies-arrayexpress

Influence of low and high force muscle activity on paralyzed muscle gene expression

Project description:We used a novel approach to study the acute effect of two frequencies of stimulation (20 Hz and 5 Hz; high and low force, respectively) on gene regulation in people with chronic paralysis. Three hours after the completion of the electrical stimulation protocol (5 Hz or 20 Hz), we sampled the vastus lateralis muscle and examined genes involved with metabolic transcription, glycolysis, oxidative phosphorylation, and mitochondria remodelling. We discovered that the 5 Hz stimulation, despite generating a lower overall force, induced a 5-6 fold increase (p<0.05) in key metabolic transcription factors including PGC-1α, NR4A3, and ABRA. Neither protocol showed a robust regulation of genes for glycolysis, oxidative phosphorylation, and mitochondria remodelling.

2015-08-01 | GSE64683 | GEO

Muscle Overload scRNA-seq

Project description:The goal of this project was to determine which mononuclear cell populations satellite cells communicate with throughout muscle during synergist ablation functional overload. Mouse plantaris muscles from tamoxifen-treated Pax7-tdT, tdT, and Pax7-DTA mice were overloaded for 4-14 days, and a subset of mononuclear cells were submitted for sc-RNA-seq.

2021-07-01 | GSE168872 | GEO

Expression data during plantaris muscle regrowth following two weeks of hind limb suspension in young adult mice

Project description:Global gene expression patterns were determined from microarray results on day 1, 3, 5, 7, 10 and 14 during plantaris muscle regrowth following two weeks of hind limb suspension in young adult mice (5 months). For each time point, one Affymetrix chip (Mouse Gene 1.0 ST) was used with 100 ng of total RNA derived from a pooled sample of the left plantaris muscle from six animals.

2014-04-16 | E-GEOD-56798 | biostudies-arrayexpress

Expression data during plantaris muscle hypertrophy induced by synergist ablation in young adult mice

Project description:Global gene expression patterns were determined from microarray results on day 1, 3, 5, 7, 10 and 14 during plantaris muscle hypertrophy induced by synergist ablation in young adult mice (5 months). For each time point, two Affymetrix chips (Mouse Gene 1.0 ST) were used with 100 ng of total RNA derived from a pooled sample of either the right or left plantaris muscles from six animals.

2013-05-21 | E-GEOD-47098 | biostudies-arrayexpress

Expression data during plantaris muscle hypertrophy induced by synergist ablation in young adult mice

2013-05-21 | GSE47098 | GEO

Expression data during plantaris muscle regrowth following two weeks of hind limb suspension in young adult mice

2014-04-16 | GSE56798 | GEO

gene expression profiling of ventilation induced muscle wasting in rat plantaris

Project description:RNASeq technology is applied to study the gene expression affected by ventilation or loading in plantaris. Non-loaded right plantaris group under short-term (6 hours to 4 days) or long-term (4.5 to 9 days) ventilation, as well as the corresponding loaded groups, are included besides the healthy control group.

2016-02-01 | E-MTAB-4228 | biostudies-arrayexpress

gene expression profiling of ventilation induced muscle wasting in rat plantaris

Project description:gene expression profiling of ventilation induced muscle wasting in rat plantaris

| PRJEB12368 | ENA