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Transcriptome sequencing of the developing internode and ligular region of maize mutant Sdw3

ABSTRACT: Transcriptome sequencing of the developing internode and ligular region of maize mutant Sdw3

PROVIDER: PRJNA524800 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8645930_1.fastq.gz	Fastqsanger.gz
	SRR8645930_2.fastq.gz	Fastqsanger.gz
	SRR8645931_1.fastq.gz	Fastqsanger.gz
	SRR8645931_2.fastq.gz	Fastqsanger.gz
	SRR8645932_1.fastq.gz	Fastqsanger.gz

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Transcriptome sequencing of the developing internode and ligular region of maize mutant Sdw3

Project description:The reduced plant height and enlarged leaf angle of Sdw3 are mainly attributed to a reduction in longitudinal cell elongation in the internode and an increase in the auricle. To investigate the transcriptome changes during internode and auricle development in Sdw3 versus N18, we performed RNA sequencing analysis.

2020-07-08 | GSE127448 | GEO

centipedegrass tiller bud and internode proteome

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Analysis of global gene expression in vegetative and reproductive tissues of maize (Zea mays) that differ in accumulation of starch and sucrose

Project description:Background. Carbon allocation between vegetative and reproductive tissues impacts cereal grain production. Despite their great agricultural importance, sink-source relationships have not been fully characterized at early reproductive stages in maize. Here we quantify the accumulation of non-structural carbohydrates and patterns of gene expression in the top internode of the stem and the female inflorescence of maize at the onset of grain filling (reproductive stage R1). Methods. Top internode stem and female inflorescence tissues of the Puma maize inbred line were collected at reproductive stage R1 (without pollination). The accumulation of non-structural carbohydrates was quantified by spectrophotometry. Global gene expression was evaluated in both tissues by RNA sequencing. Results. At reproductive stage R1, the maize female inflorescence accumulates non-structural carbohydrates, notably starch, at higher levels than the top internode of the stem. Gene expression analysis identified 491 genes to be differentially expressed between the female inflorescence and the stem top internode. Gene ontology classification of differentially expressed genes showed enrichment for sucrose synthesis, the light-dependent reactions of photosynthesis, and transmembrane transporters. Our results suggest that sugar transporters play a key role in sugar partitioning in the maize stem and reveal previously uncharacterized differences between the female inflorescence and the top internode of the stem at early reproductive stages.

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Comparision of an AGCVIII kinase mutant with wild type in sorghum internode tissues

Project description:An AGCVIII kinase (Dw2) regulates sorghum stem internode growth, but the underlying mechanism and signaling network are unknown. Here we provide evidence that mutation of Dw2 reduces cell proliferation in internode intercalary meristems, inhibits endocytosis, and alters the distribution of heteroxylan and mixed linkage glucan in cell walls. Phosphoproteomic analysis showed that Dw2 signaling influences the phosphorylation of proteins involved in lipid signaling (PLDδ), endomembrane trafficking, hormone, light and receptor signaling, and photosynthesis. Together

2020-11-20 | PXD020160 | Pride

Comparative transcriptome analysis of abscisic acid (ABA) deficient mutant (vp5) and normal maize (Vp5) embryos during seed maturation

Project description:Identification of key genes and pathways regulated by phytohormone ABA during maize seed maturation, using the ABA synthesis-deficient mutant (vp5) and regular maize (Vp5) developing embryos.

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Project description:S. frugiperda fed with bx3 maize mutant and its control genotype B73 AND fed with bx1 maize mutant and its control genotype H88. The bx1 mutant is derived from the insertion of a Mu element within the Bx1 gene in a H88 genetic background (Hamilton 1964). Similarly, the bx3 mutant was obtained in the genotype B73 by insertion of a Mu element in the Bx3 gene (Frey, Chomet et al. 1997).

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Gene expression in developing ears of ra3;tpp4 maize mutants compared to wild-type

Project description:We performed mRNA-seq on developing ears (2-3 mm length) of ra3;tpp4 maize mutants compared to segregating wild-types.

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Transcriptomic analysis of maize developing earshoot, IBMRIL X IHP1 hybrid

Project description:Maize earshoot is a metabolic sink espcially related to nitrogen metabolism. Studies on the transcriptomic and metabolic changes occuring in earshoot can provide interesting answers about the nitrogen metabolic potential of the maize variety under study. Intermated B73 X Mo17 recombinant inbred lines (IBMRIL) belong to a predominantly used mapping population. Illinois high protein (IHP) line is a metabolic extreme with respect to nitrogen mebolism with around 30% seed protein. Developing earshoots from this genotype grown at nitrogen-deficient and nitrogen-sufficient conditions were sampled, processed and analyzed through microarray technology. IBMRIL population was crossed to IHP to find the allelic interaction related to nitrogen metabolism. Microarray experiment was conducted using the developing earshoots from IBMRIL X IHP1 lines that deffered in their grain yield significantly. Keywords: Yield response

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