Project description:SPY is an O-linked N-acetylglucosamine transferase involved in many signaling and developmental processes in Arabidopsis. SPY also has a role in root development. In the spy mutant, a middle cortex is produced prematurely. To understand how SPY regulates root cortex proliferation, we sought to identify the genes affected by the spy mutation in the root.
Project description:Dictyostelium discoideum O-Ser and O-Thr fucosylated proteins from WT, SPY-OE and SPY-KO vegetative cells were immunoprecipitated using anti O-Ser and anti O-Thr fucose polyclonal antibodies in 3 Technical Replicates, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation), and SPY_OE (mutant, SPY overexpressor strain).
