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Next Generation Sequencing Facilitates Quantitative Analysis of SCs in mice exposure to exercise

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of SCs in mice exposure to exercise

PROVIDER: PRJNA528405 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8759690_1.fastq.gz	Fastqsanger.gz
	SRR8759690_2.fastq.gz	Fastqsanger.gz
	SRR8759691_1.fastq.gz	Fastqsanger.gz
	SRR8759691_2.fastq.gz	Fastqsanger.gz
	SRR8759684_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing Facilitates Quantitative Analysis of SCs in mice exposure to exercise

Project description:Purpose:To identify genes and the molecular pathways involved in the regulation of muscle satellite cells (SCs) in mice exposure to running exercise, we performed RNA-Sequence of SCs in untrained (Con) and trained mice (Exe). Methods: mRNA profiles of SCs in untrained (Con) and trained mice (Exe) (running exercise, 4 days/week, 30 min/day at 12 m/min) were generated by deep sequencing, in quadruplicate, using Illumina HiSeq 2000. Results:Using an optimized data analysis workflow, we mapped about 9 million sequence reads per sample to the mouse genome (build mm10) and identified 9031 transcripts in SCs (the control and exercise group) with BWA workflow. Conclusions: Our results represent the first detailed analysis of SCs transcriptomes in mice exposure to running exercise and found that exercise regulated multiple mRNA pathways in SCs.

2020-06-08 | GSE128651 | GEO

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