Modeling protein destiny in developing tomato fruit
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA528656 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR8772140_1.fastq.gz | Fastqsanger.gz | |||
| SRR8772140_2.fastq.gz | Fastqsanger.gz | |||
| SRR8772150_1.fastq.gz | Fastqsanger.gz | |||
| SRR8772150_2.fastq.gz | Fastqsanger.gz | |||
| SRR8772160_1.fastq.gz | Fastqsanger.gz |
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Project description:Proteomics and transcriptomics data of tomato fruits at 9 developmental stages were used to calculate with a mathematical model the rate constants of synthesis and degradation for over 1,000 proteins.
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Project description:To evaluate the role of seeds in fruit quality, we induced parthenocarpy in tomato by regulating ovule-specific auxin synthesis or responsiveness using the INO promoter from A. thaliana, which is expressed in the outer layer of the integuments during early stages of ovule development. We compared these to fruit where the same coding regions were expressed from the DeFH9 promoter which is expressed in carpel tissues during early stages of ovule development. Expression of auxin synthesis or responsiveness genes by both of these promoters produced seedless parthenocarpic tomato fruit. We compared fruit samples using the Affymetrix tomato GeneChip (GPL4741) to determine how gene regulation and expression differed between wild-type and transgenic fruit. Experiment Overall Design: Wild-type fruit with seeds was compared with transgenic lines INO-IaaM, DefH9-IaaM, INO-RolB, and DefH9-RolB. To find genes with seed-specific expression, we also compared the control with wild-type fruit from which seeds had been manually removed. We had three biological replicates for each treatment and control except DefH9-RolB, for which only two replicates were available. Each CEL file from the microarray represents one plant from each line.
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