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Rainbow trout IgM+ B cells preferentially respond to thymus-independent antigens but are activated by CD40L

ABSTRACT: Rainbow trout IgM+ B cells preferentially respond to thymus-independent antigens but are activated by CD40L

PROVIDER: PRJNA530062 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8820580.fastq.gz	Fastqsanger.gz
	SRR8820572.fastq.gz	Fastqsanger.gz
	SRR8820573.fastq.gz	Fastqsanger.gz
	SRR8820574.fastq.gz	Fastqsanger.gz
	SRR8820575.fastq.gz	Fastqsanger.gz

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Rainbow trout IgM+ B cells preferentially respond to thymus-independent antigens but are activated by CD40L

Project description:To further investigate the differential effects exerted by TD and TI-1 antigens on trout IgM+ B cells, RNA sequencing (RNA-seq) was performed on FACS isolated splenic IgM+ B cells that had been stimulated with TNP-KLH or TNP-LPS for 24 h, or on control unstimulated splenic IgM+ B cells

2019-12-10 | GSE129092 | GEO

Rainbow Trout IgM<sup>+</sup> B Cells Preferentially Respond to Thymus-Independent Antigens but Are Activated by CD40L.

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The T cell-DC CD40L-CD40 axis, but not platelet CD40L, drives atherosclerosis

Project description:Atherosclerosis, the underlying vascular cause of cardiovascular disease, has a strong inflammatory component. The co-stimulatory CD40-CD40 ligand (CD40L) signaling axis is a pivotal regulator of immune responses in atherosclerosis. However, therapeutic long-term inhibition of CD40L will severely compromise the immune system making it a non-viable treatment option. To circumvent this issue, cell-specific inhibition may present a better approach to target the CD40-CD40L axis. Therefore, we generated T cell and platelet-specific knockout mice for CD40L and apolipoprotein E, which were aged for 28 weeks to study their effects on immune status and atherosclerosis. Here, we show that T cell specific deficiency in CD40L signaling reduced plaque progression through hampered Th1 polarization as well as reduced antigen-dependent proliferation and oxLDL IgM production. DC-specific CD40 deficient mice displayed a similar phenotype. Platelet-specific CD40L deficiency, however, failed to decrease atherosclerosis, but ameliorated atherothrombosis. Together, our results illuminate the divergent cell-specific mechanisms of CD40-CD40L signaling in atherosclerosis, which may lead to advances in targeted therapies.

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RNA-seq analysis of splenic IgG1 or IgM plasma cells

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Project description:To assess the nature of CD8+CD40L+ memory Tcells, we compared the gene expression to CD8+CD40L- and CD4+ counterparts, and found similarities in expression of genes encoding cytokines. PBMCs were isolated from five healthy donors. The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort.

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Expression data from Human CD40L+ Tcells

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Transcriptomic analysis of human naive B cells stimulated by CD40L, CpG, anti-IgM IgG, IL-4 or combinations

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Transcriptomic profiling and IgM and IgT repertoire dynamics in rainbow trout gills following primary and secondary challenge with Lactococcus petauri

Project description:Fish mucosal tissues lack the organized lymphoid structures present in mammals. Although this is also true for the gills, with the gill-associated lymphoid tissue (GIALT) being mainly composed of B and T cells scattered throughout the epithelium and the lamellae, in some species such as rainbow trout (Oncorhynchus mykiss) or Atlantic salmon (Salmo salar), a more organized structure designated as the interbranchial lymphoid tissue (ILT) is also identified. Yet, although many studies have investigated the immune response of the gill to stimulation or pathogen encounter, which have revealed the activation of both innate and adaptive immune elements, how the gills respond to secondary stimulation or whether memory responses are locally orchestrated is still unknown. To provide insights into this matter, in the current study, we have infected rainbow trout by bath with Lactococcus petauri and then re-infected or mock-infected the survivor fish as well as mock-infected controls. At day 4 after the last infection, the fish were sacrificed and gills used to perform an RNAseq transcriptomic analysis and an IgM and IgT repertoire analysis. The number of cells secreting total and specific IgM in gills was also established by ELIspot. Our results demonstrate that memory responses are locally organized in the rainbow trout gills, given that re-infected fish did not up-regulate genes related to inflammation (as happens in the primary infection), but preferentially modified genes related to adaptive immunity, mainly to B cell function, experiencing a significant IgM and IgT clonal expansion. Accordingly, re-infected fish produced increased levels of specific IgM both locally and in serum, although no clonal selection was apparent. These findings advance our understanding of mucosal immunological memory in teleost fish and reveal the gills as a site of localized adaptive immune regulation during secondary responses.

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Expression data from Human CD40L+ Tcells

2013-08-05 | GSE44126 | GEO

RNA-seq analysis of germinal center-derived splenic IgG1 or IgM plasma cells

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