Project description:To analyze the gain and lost of genes in Aggregatibacter actinomycetemcomitans during short-term persistent infection in the same host
Project description:To analyze the gain and lost of genes in Aggregatibacter actinomycetemcomitans during short-term persistent infection in the same host The microarray was used to compare gene contents of 4 different pairs of Aggregatibacter actinomycetemcomitans strains (SCC393 vs A160, SCC1398 vs SCC4092, SCC2302 vs AAS4a, and S23A vs I23C), where each pair was isolated from an individual over time. Two biological duplicates were carried out for each strain.
Project description:Transcriptional profiling was utilized to define the biological pathways of gingival epithelial cells modulated by co-culture with the oral pathogenic Porphyromonas gingivalis and Aggregatibacter (formerly actinobacillus) actinomycetemcomitans. We used microarrays to detail the global programme of gene expression underlying infection and identified distinct classes of up- and down-regulated genes during this process. Keywords: infection state
Project description:To determine the biological mechanisms underlying a dampened immune response to Porphyromonas gingivalis, as compared to Aggregatibacter actinomycetemcomitans challenge, we infected primary BMDCs with either pathogen or left uninfected Total RNA from uninfected BMDCs compared to BMDCs infected with either Aggregatibacter actinomycetemcomitans or Porphyromonas gingivalis
Project description:To determine the biological mechanisms underlying a dampened immune response to Porphyromonas gingivalis, as compared to Aggregatibacter actinomycetemcomitans challenge, we infected primary BMDCs with either pathogen or left uninfected
Project description:Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen associated with aggressive forms of periodontitis, and with endocarditis. Outer membrane vesicles (OMVs) released by this species have been demonstrated to deliver effector proteins such as cytolethal distending toxin (CDT) and leukotoxin (LtxA) into human host cells, and to act as triggers of innate immunity upon carriage of NOD1- and NOD2-active pathogen-associated molecular patterns (PAMPs). To improve our understanding of the pathogenicity-associated functions that A. actinomycetemcomitans exports via OMVs, we have used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to characterize the OMV-associated proteome of a rough-colony type clinical isolate, strain 173 (serotype e). This yielded identification of 151 proteins overlapping in three out of four independent experiments, using density gradient-purified OMVs. Further to confirming the vesicle-associated release of LtxA, and of several proteins earlier demonstrated to act as immunoreactive antigens in the human host, we identified numerous additional putative virulence-related proteins in the A. actinomycetemcomitans OMV proteome, including proteins involved in immune evasion, drug targeting, and iron/nutrient acquisition. In summary our findings are consistent with an OMV-associated proteome that exhibits several offensive and defensive functions, and they provide a comprehensive basis to further disclose roles of A. actinomycetemcomitans OMVs in periodontal and systemic disease.
Project description:To characterize the differences in pattern of gene expression between different pairs of Aggregatibacter actinomycetemcomitans strains (SCC393 vs A160, SCC1398 vs SCC4092, and S23A vs I23C), where each pair was isolated from an individual over time. The microarray was used to examine the transcriptome of Aggregatibacter actinomycetemcomitans strains SCC393, A160, SCC1398, SCC4092, S23A, and I23C, where biological triplicates were produced for strains SCC1398, SCC4092, S23A, and I23C and biological duplicates were produced for strains SCC393, and A160
Project description:To characterize the differences in pattern of gene expression between different pairs of Aggregatibacter actinomycetemcomitans strains (SCC393 vs A160, SCC1398 vs SCC4092, and S23A vs I23C), where each pair was isolated from an individual over time.
Project description:Aggregatibacter actinomycetemcomitans is a Gram-negative facultative anaerobe and is associated with periodontal disease. A two-component system ArcAB plays a crucial role in adaptation to several conditions, but its function in A. actinomycetemcomitans remains unclear. To identify the genes regulated by the ArcA response regulator in A. actinomycetemcomitans, differentially expressed genes were analyzed using the wild-type strain NUM4039 and its isogenic mutant of arcA. The obtained data indicated that 68 genes were differentially expressed with 40 genes upregulated more than 2-fold and 28 genes downregulated more than 2-fold.