Project description:Proteomic analysis of the chain elongating bacteria Pseudoramibacter alactolyticus and Anaerostipes caccae during lactate fermentation with supplemented acetic acetate. The impact of acetate absence on P. alactolyticus soluble proteome was also evaluated. (Principal investigator: Christopher Lawson, Toronto, ON, Canada).
Project description:Whole-Genome Sequences of Streptococcus alactolyticus Strain An1F4 and Escherichia coli Strains Ae3A3 and Ae3B3, Isolated from Feces of Domestic Pigs
Project description:This study looks at the gene expression of two chain-elongating bacteria, Pseudoramibacter alactolyticus and Megasphaera elsdenii, in monoculture and in coculture with anaerobic gut fungi (Neocallimastix lanati). P. alactolyticus in particular has appeared stable in synthetic cocultures with anaerobic fungi based on metabolic output, and this RNA-seq study was designed to further assess that stability. Additionally, chain-elongating bacteria can use fungal fermentation products to generate short- and medium-chain fatty acids, and this study was employed to see how genes involved in this process (reverse beta oxidation) were regulated in coculture compared to monoculture.