Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Physiological and transcriptional response of Candida parapsilosis to exogenous tyrosol

ABSTRACT: Physiological and transcriptional response of Candida parapsilosis to exogenous tyrosol

PROVIDER: PRJNA531086 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8858170.fastq.gz	Fastqsanger.gz
	SRR8858171.fastq.gz	Fastqsanger.gz
	SRR8858172.fastq.gz	Fastqsanger.gz
	SRR8858173.fastq.gz	Fastqsanger.gz
	SRR8858174.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Physiological and transcriptional response of Candida parapsilosis to exogenous tyrosol

Project description:Tyrosol plays a key role in fungal morphogenesis and biofilm development. Also, it has a remarkable antifungal effect at supraphysiological concentrations. However, the background of the antifungal effect remains unknown, especially in the case of non-albicans Candida species such as Candida parapsilosis We examined the effect of tyrosol on the growth, adhesion, redox homeostasis, virulence, as well as on fluconazole susceptibility. To gain further insights into the physiological consequences of tyrosol treatment we also determined the caused genome-wide gene expression changes using RNA-Seq. Fifteen mM tyrosol caused significant growth inhibition within two hours of the addition of tyrosol, while the adhesion of yeast cells was not affected. Tyrosol increased the production of reactive oxygen species remarkably as revealed by the dichlorofluorescein-test, and it was associated with elevated superoxide dismutase, glutathione peroxidase, and catalase activities. The interaction between fluconazole and tyrosol was antagonistic. Tyrosol exposure resulted in 261 and 181 differentially expressed genes with at least a 1.5-fold increase or decrease in expression, respectively, were selected for further study. Genes involved in ribosome biogenesis showed down-regulation, while genes related to oxidative stress response, and ethanol fermentation were up-regulated. In addition, tyrosol treatment up-regulated the expression of efflux pump genes including MDR1 and CDR1 and down-regulated the FAD2 and FAD3 virulence genes involved in desaturated fatty acid formation. Our data demonstrate that exogenous tyrosol significantly affects the physiology and gene expression of C. parapsilosis, which could contribute to the development of treatments targeting quorum-sensing in the future.

2019-08-23 | GSE129372 | GEO

Transcriptional response of an azole-resistant Candida parapsilosis isolate.

Project description:This SuperSeries is composed of the following subset Series: GSE27405: Transcriptional response of an azole-resistant Candida parapsilosis isolate [fluconazole]. GSE27407: Transcriptional response of an azole-resistant Candida parapsilosis isolate [posaconazole]. GSE27408: Transcriptional response of an azole-resistant Candida parapsilosis isolate [voriconazole]. Refer to individual Series

2011-04-18 | E-GEOD-27409 | biostudies-arrayexpress

Transcriptional profile of Candida parapsilosis bcr1 knockout.

Project description:Whole genome microarrays were used to compare the transcriptional profile of Candida parapsilosis bcr1 knockout to wild type cells.

2011-11-04 | GSE33461 | GEO

Role of Hac1 in unfolded protein response in Candida parapsilosis

Project description:Investigation of the role of HAC1 using transcriptional profiling of Candida parapsilosis treated with 5 mM dithiothreitol Gene expression of wild type and hac1 deletion strains were compared during growth in YPD only and growth in YPD with 5 mM DTT

2018-10-08 | GSE120094 | GEO

Centromere locations and structure in Candida parapsilosis.

Project description:Investigation of centromeres in the pathogenic yeast Candida parapsilosis, shows that the location of two centromeres are polymorphic within this species. The centromeres consist of large inverted repeats (IRs), surrounding unique sequences. New (neo) centromeres have emerged in one C. parapsilosis isolate even though the original CEN location is undamaged. The neocentromeres do not contain IRs, and have no obvious sequence features.

2020-04-17 | GSE136854 | GEO

Hypoxia and biofilms in Candida parapsilosis

Project description:This SuperSeries is composed of the following subset Series: GSE13717: Transcriptional profile of Candida parapsilosis in SD media GSE13722: Transcriptional response of Candida parapsilosis in low oxygen (hypoxic) conditions in SD media Refer to individual Series

2010-05-06 | E-GEOD-13832 | biostudies-arrayexpress

Transcriptional landscape of the pathogenic yeast Candida parapsilosis

Project description:This SuperSeries is composed of the following subset Series: GSE32712: Transcriptional profile of Candida parapsilosis CLIB214 21% Oxygen (normoxia) versus at 1% oxygen (hypoxia). GSE32713: Transcriptional profile of Candida parapsilosis CLIB214 versus UPC2 delete, both at 1% oxygen (hypoxia) GSE32714: Transcriptional landscape of Candida parapsilosis Refer to individual Series

2011-11-30 | E-GEOD-32716 | biostudies-arrayexpress

Homo sapiens whole blood infected with Candida spp. (here: Candida parapsilosis)

Project description:Homo sapiens fresh whole blood was infected with Candida parapsilosis. RNA-pool of both species extracted at 0min (control), 15, 30, 60, 120, 240 min. Samples are rRNA depleted. Measurement of Homo sapiens gene expression.

2018-12-26 | GSE114177 | GEO

Transcriptional profile of Candida parapsilosis CLIB214 culture in low iron conditions

Project description:Whole genome microarrays were used to generate the transcriptional profile of Candida parapsilosis in low iron conditions

2011-11-04 | GSE33462 | GEO

Comparative phenotypic analysis of the major fungal pathogens Candida parapsilosis and Candida albicans

Project description:Abstract: Candida parapsilosis and Candida albicans are human fungal pathogens that belong to the CUG clade in the Saccharomycotina. In contrast to C. albicans, relatively little is known about the virulence properties of C. parapsilosis, a pathogen particularly associated with infections of premature neonates. We describe here the construction of >200 C. parapsilosis strains carrying double allele deletions of transcription factors, protein kinases and species-specific genes. Two independent deletions were constructed for each target gene. Growth in > 40 conditions was tested, including carbon source, temperature, and the presence of antifungal drugs. The phenotypes were compared to C. albicans strains with deletions of orthologous transcription factors. We found that many phenotypes are shared between the two species, such as the role of Upc2 as a regulator of azole resistance. Others are unique. For example, Cph2 plays a role in the hypoxic response in C. parapsilosis and not in C. albicans. We found extensive divergence between the biofilm regulators of the two species. We identified 7 transcription factors and one protein kinase that are required for biofilm development in C. parapsilosis. Only three (Efg1, Bcr1, and Ace2) have similar effects on C. albicans biofilms, whereas Cph2, Czf1, Gzf3 and Ume6 have major roles in C. parapsilosis only. In addition, two transcription factors (Brg1 and Tec1) with well-characterized roles in biofilm formation in C. albicans do not have the same function in C. parapsilosis. We also compared the transcription profile of C. parapsilosis and C. albicans biofilms. Our analysis suggests the processes shared between the two species are predominantly metabolic.

2014-07-21 | GSE57451 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data