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Transcriptome analysis of MAZR and/or Runx3-deficient cytotoxic T lymphocytes

ABSTRACT: Transcriptome analysis of MAZR and/or Runx3-deficient cytotoxic T lymphocytes

PROVIDER: PRJNA532724 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8893040.fastq.gz	Fastqsanger.gz
	SRR8893031.fastq.gz	Fastqsanger.gz
	SRR8893041.fastq.gz	Fastqsanger.gz
	SRR8893032.fastq.gz	Fastqsanger.gz
	SRR8893042.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of MAZR and/or Runx3-deficient cytotoxic T lymphocytes

Project description:We investigated transcriptional changes in MAZR-, Runx3- and MAZR/Runx3-deficient cytotoxic T lymphocytes (CTLs). This analysis revealed that MAZR plays a compensatory role in the Runx3-dependent transcriptional program of CTL differentiation.

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Runx3-mediated transcriptional program in cytotoxic lymphocytes.

Project description:The transcription factor Runx3 is highly expressed in CD8(+) T and NK cytotoxic lymphocytes and is required for their effective activation and proliferation but molecular insights into the transcription program regulated by Runx3 in these cells are still missing. Using Runx3-ChIP-seq and transcriptome analysis of wild type vs. Runx3(-/-) primary cells we have now identified Runx3-regulated genes in the two cell types at both resting and IL-2-activated states. Runx3-bound genomic regions in both cell types were distantly located relative to gene transcription start sites and were enriched for RUNX and ETS motifs. Bound genomic regions significantly overlapped T-bet and p300-bound enhancer regions in Runx3-expressing Th1 helper cells. Compared to resting cells, IL-2-activated CD8(+) T and NK cells contain three times more Runx3-regulated genes that are common to both cell types. Functional annotation of shared CD8(+) T and NK Runx3-regulated genes revealed enrichment for immune-associated terms including lymphocyte activation, proliferation, cytotoxicity, migration and cytokine production, highlighting the role of Runx3 in CD8(+) T and NK activated cells.

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Precursors of human CD4+ cytotoxic T lymphocytes identified by single-cell transcriptome analysis

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Protein palmitoylation and sphingolipid metabolism cooperatively 1 control regulated exocytosis in cytotoxic lymphocytes

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In-depth analysis of the phosphoprotein landscape of cytotoxic T lymphocytes and its regulation by cytokines

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Protein palmitoylation and sphingolipid metabolism control regulated exocytosis in cytotoxic lymphocytes

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