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Differentially expressed genes in livers and abdominal fats between normal- and high-fat diet-fed dwarf broilers

ABSTRACT: Differentially expressed genes in livers and abdominal fats between normal- and high-fat diet-fed dwarf broilers

PROVIDER: PRJNA532926 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8902630.fastq.gz	Fastqsanger.gz
	SRR8902631.fastq.gz	Fastqsanger.gz
	SRR8902632.fastq.gz	Fastqsanger.gz
	SRR8902633.fastq.gz	Fastqsanger.gz
	SRR8902634.fastq.gz	Fastqsanger.gz

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Differentially expressed genes in livers and abdominal fats between normal- and high-fat diet-fed dwarf broilers

Project description:To investigate genes involved in abdominal fat deposition and fat metabolism of broilers with dw gene, we used highthroughput sequencing to detect the differentially expressed genes in livers and abdominal fats of dwarf broilers which were fed with a normal diet and a high-fat diet, respectively. The broilers began to fed with a normal or a high-fat diet in 1-week-old. After 7 weeks, the broilers were be executed and the livers and abdominal fats were used to extracted total RNAs. Finally, the total RNAs were be sequenced used BGISEQ-500 platform.

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Differentially expressed genes in livers and abdominal fats between normal- and high-fat diet-fed chickens

Project description:To investigate genes involved in abdominal fat deposition and fat metabolism of broilers, we used highthroughput sequencing to detect the differentially expressed genes in livers and abdominal fats of broilers which were fed with a normal diet and a high-fat diet, respectively. The broilers began to fed with a normal or a high-fat diet in 1-week-old. After 7 weeks, the broilers were be executed and the livers and abdominal fats were used to extracted total RNAs. Finally, the total RNAs were be sequenced used BGISEQ-500 platform.

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Transcription profiling of livers from aged and young Snell dwarf mice and their wild type controls to identify genetic signatures.

Project description:To compare and contrast genetic signatures from livers of young and aged Snell dwarf mice with their wild type controls. SUBMITTER_CITATION: W. H. Boylston, James H. DeFord, John Papaconstantinou (2006) Identification of longevity-associated genes in long-lived Snell and Ames dwarf mice Age 28:125-144 Experiment Overall Design: 25 micrograms of liver RNA was isolated from each of 6 aged (3 dwarf, 3 wild type) and 8 young (4 dwarf, 4 wild type) mice for use in hybridization of Affymetrix gene chips according to Affymetrix protocols.

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Project description:The lean and fat broilers were derived from the 21st generation of the Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF). In brief, broilers in the two lines (lean line and fat line) of the NEAUHLF have the same Arbor Acres ancestry, but were divergently selected by the concentration of very low-density lipoprotein and abdominal fat percentage (AFP) at 7 weeks old. After 21 generations of selection, there is over a 10-fold difference in abdominal fat percentage between the two lines, representing a classical obese-lean-study model. At age of 7 weeks, ten male individuals of each line were selected randomly to investigate the interactions between gut microbiome, host gene expression and obesity.

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