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Small RNA-seq from FPLC-fractionated leaf and flower crude extracts

ABSTRACT: Small RNA-seq from FPLC-fractionated leaf and flower crude extracts

PROVIDER: PRJNA540255 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8984470.fastq.gz	Fastqsanger.gz
	SRR8984480.fastq.gz	Fastqsanger.gz
	SRR8984471.fastq.gz	Fastqsanger.gz
	SRR8984481.fastq.gz	Fastqsanger.gz
	SRR8984472.fastq.gz	Fastqsanger.gz

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Small RNA-seq from FPLC-fractionated leaf and flower crude extracts

Project description:Background: RNA interference (RNAi) is an indispensable regulatory mechanism governing developmental processes and stress responses via sequence-specific control of target RNAs mediated by the action of small, 20–24-nt-long, non-coding regulatory (s)RNAs such as micro (mi) and small interfering (si) RNAs. Biogenesis and sorting of miRNAs into ARGONAUTE (AGO) proteins are intensively investigated, however very few information is available about the presence and distribution of distinct sRNA pools in plant cells. Results: High-throughput sequencing of size-separated sRNA pools of plant crude extracts revealed that the majority of the canonical miRNAs were associated with high molecular weight RNA-induced silencing complexes co-migrating with AGO1 (HMW RISC). In contrast, the majority of 24-nt-long siRNAs were found in association with low molecular weight complexes co-migrating with AGO4 (LMW RISC). Intriguingly, we identified a large set of sRNAs in the cytoplasm, including mature miRNA sequences, in the low molecular size range corresponding to protein-unbound sRNAs. By comparing the RISC-loaded and protein-unbound pools of miRNAs, we identified miRNAs with highly different loading efficiencies. Investigation of some selected miRNAs in a transient expression system validated this finding. We also showed that the availability of RISCs is a limiting factor determining the loading efficiency of miRNAs. Conclusion: Our data reveal the existence of a regulatory checkpoint, likely controlled by information carried by the diverse miRNA precursors, determining the RISC-loading efficiencies of various miRNAs by sorting only a subset of the produced miRNAs into the biologically active RISCs.

2019-07-29 | GSE130431 | GEO

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Lychnophora plant family crude extracts

Project description:LC-MS/MS of plant crude extracts from Brazil. Species of Lychnophora family.

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Jordan Plants - AB - Crude and Extracts

Project description:Jordan Plants - AB - Crude and Extracts - Ethyl Acetate

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Anti-HIV Activity and Immunomodulatory Properties of Fractionated Crude Extracts of Alternaria alternata

Project description: Not available

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Jordan Plants - PA - Crude and Extracts

Project description:Jordan Plants - Pistacia atlantica - Ethyl Acetate Extracts - Crude and Fractional

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Jordan Plants - PS - Crude and Extracts

Project description:Jordan Plants - Phlomis syriaca - Ethyl Acetate Extracts - Crude and Fractional

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Jordan Plants - LD - Crude and Extracts

Project description:Jordan Plants - Lavandula dentata - Ethyl Acetate Extracts - Crude and Fractional

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High-throughput pyrosequencing of endogenous small RNAs from Arabidopsis thaliana wild-type and polIV mutants

Project description:Comparison of the endogenous small RNA content of Arabidopsis flower bud tissue: wild type vs. mutants in polIV pathways Keywords: High throughput 454 small RNA sequencing. Size fractionated small RNA from total RNA extracts was ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to 454 high throughput pyrosequencing. Please see www.454.com for details of the sequencing technology.

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