Ontology highlight
ABSTRACT:
PROVIDER: PRJNA540400 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR8988650_1.fastq.gz | Fastqsanger.gz | |||
| SRR8988650_2.fastq.gz | Fastqsanger.gz | |||
| SRR8988660_1.fastq.gz | Fastqsanger.gz | |||
| SRR8988660_2.fastq.gz | Fastqsanger.gz | |||
| SRR8988670_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 54 |
Similar Datasets
Project description:BACKGROUND: Social insects, such as honey bees, use molecular, physiological and behavioral responses to combat pathogens and parasites. The honey bee genome contains all of the canonical insect immune response pathways, and several studies have demonstrated that pathogens can activate expression of immune effectors. Honey bees also use behavioral responses, termed social immunity, to collectively defend their hives from pathogens and parasites. These responses include hygienic behavior (where workers remove diseased brood) and allo-grooming (where workers remove ectoparasites from nestmates). We have previously demonstrated that immunostimulation causes changes in the cuticular hydrocarbon profiles of workers, which results in altered worker-worker social interactions. Thus, cuticular hydrocarbons may enable workers to identify sick nestmates, and adjust their behavior in response. Here, we test the specificity of behavioral, chemical and genomic responses to immunostimulation by challenging workers with a panel of different immune stimulants (saline, Sephadex beads and Gram-negative bacteria E. coli). RESULTS: While only bacteria-injected bees elicited altered behavioral responses from healthy nestmates compared to controls, all treatments resulted in significant changes in cuticular hydrocarbon profiles. Immunostimulation caused significant changes in expression of hundreds of genes, the majority of which have not been identified as members of the canonical immune response pathways. Furthermore, several new candidate genes that may play a role in cuticular hydrocarbon biosynthesis were identified. Finally, we identified common genes regulated by pathogen challenge in honey bees and other insects, suggesting that immune responses are conserved at the molecular level. CONCLUSIONS: These studies suggest that honey bee genomic responses to immunostimulation are substantially broader than expected, and may mediate the behavioral changes associated with social immunity by orchestrating changes in chemical signaling.
2012-12-02 | E-MEXP-3708 | biostudies-arrayexpress
Project description:<p>One of the main challenges of the socially parasitic mode of life is bypassing the host's recognition ability, which ensures that altruistic behaviour is directed towards related individuals. Various chemical strategies have evolved to achieve this goal. The most widespread, used also by the obligate slave-making ants, is camouflage or mimicry of colony odour encoded in cuticular hydrocarbon (CHC) composition. However, recent studies have shown that facultative slave-makers employ a different strategy: they manipulate the slaves' recognition labels to make them resemble the parasite's CHC profile. We examined the limitations of this strategy by focusing on incipient F. sanguinea colonies, where slaves are the majority. Our study revealed that callow F. sanguinea ants initially suppress their species-specific odour profile, which develops gradually over time accompanied by an increase of CHC amount per surface area in slave-maker workers. This allows the slaves to familiarise themselves with the parasite's CHC. We found that callow ants produce lower amounts of CHC, and the relative abundance of certain compounds differs from what is observed in older ants. Additionally, preimaginal stages of F. sanguinea ants acquire CHC from the slaves, which are later incorporated into the imagines’ recognition labels. These findings support the proposition that the parasite's manipulation strategy is limited by the slaves' learning capacity, which is necessary to maintain colony cohesion. They also shed light on the selective pressures that might have led to the evolution of chemical mimicry in mature obligate slave-maker colonies.</p>
2026-05-19 | MTBLS13712 | MetaboLights
Project description:In the fire ant Solenopsis invicta, a colony queen number is determined by the founding queen's genotypes at the 13 Mb supergene with the non-recombining variants SB and Sb. Single-queen colonies are always headed by SB/SB queens while multiple-queens colonies are always headed by SB/Sb queens. The two variants of the supergene, SB and Sb are completely linked to the two alleles (B and b) of the gene Gp-9. SB/SB and SB/Sb queens differ in many physiological traits including their maturation rate and odor. To explain why SB/SB and SB/Sb queens have different odors, and why SB/SB virgins mature faster and accumulate more fat, we measured expression of ~6000 genes in virgin queens 1 and 11 days after eclosion and in reproductive queens. Keywords: fire ants, Solenopsis invicta, Supergene, queen, Gp-9, social form, maturation, fat storage, queen odor, cuticular hydrocarbon, worker discrimination, monogyne, polygyne, transposon, chemical signaling
2012-11-07 | GSE42062 | GEO
Project description:The differential production of pheromones is a major barrier to mating between species in Drosophila. Individuals from morphologically similar sister species can produce different sets of cuticular hydrocarbons that allow potential mates to identify them as a suitable partner. In order to elucidate cis-regulatory mechanisms behind speciation, we looked for allele-specific expression in hydrocarbon-producing oenocytes from F1 hybrids of the sister species D. simulans and D. sechellia. By focusing on cis-regulatory changes specific to female oenocytes, we rapidly identified a small number of candidate genes. Oour RNA-seq approach proved to be far more efficient than QTL mapping in identifying candidate genes, and it can be used to pinpoint the genetic basis for a wide range of traits differing due to cis-regulatory divergence between any interfertile species.
2018-05-16 | GSE114478 | GEO
Project description:Caryopses of barley grains become firmly adhered to the glumes of the husk during grain development through a cuticular cementing layer on the caryopsis surface. The quality of this attachment varies among cultivars, with poor quality adhesion causing “skinning”, an economically significant grain quality defect for the malting industry. Malting cultivars encompassing a range of husk adhesion qualities were grown under a misting treatment known to induce skinning. Changes in gene expression during adhesion development were examined with a custom barley microarray. The abundance of transcripts involved early in cuticular lipid biosynthesis, including acetyl-CoA carboxylase, and all four members of the fatty acid elongase complex of enzymes was significantly higher early in caryopsis development than later. Members of the subsequent cuticular lipid biosynthetic pathways were also higher early in development including the decarbonylation and reductive pathways, and sterol biosynthesis.
2019-05-02 | E-MTAB-7429 | biostudies-arrayexpress
Project description:Our study found that the non-sex specific Fru isoform (FruCOM) is necessary for pheromone biosynthesis in hepatocyte-like oenocytes for sexual attraction. Loss of FruCOM in oenocytes resulted in adults with reduced levels of the cuticular hydrocarbons (CHCs), including sex pheromones, and show altered sexual attraction and reduced cuticular hydrophobicity. To further determine the downstream related mechanism, we performed bulk RNAseq analysis on the oenocyte of Drosophila melanogaster.
2023-03-25 | GSE227895 | GEO
Project description:This SuperSeries is composed of the following subset Series: GSE31344: smRNA sequencing of queen and virgin queen of two ants: Camponotus floridanus and Harpegnathos saltator GSE31346: Transcriptome sequencing of queen and virgin queen of two ants: Camponotus floridanus and Harpegnathos saltator GSE31576: Single base resolution methylome of two ants: Camponotus floridanus and Harpegnathos saltator Refer to individual Series
2012-08-08 | E-GEOD-31577 | biostudies-arrayexpress
Project description:The cuticular wax, commonly known as fruit bloom, plays a crucial role in the postharvest quality, shelf-life, and stress resistance of blueberries. However, the transcriptional regulatory networks underlying its formation during fruit maturation remain largely unknown. In this study, we performed comparative transcriptome sequencing (RNA-seq) on blueberry fruits at three critical developmental stages: the early green fruit stage (stage 1), the color-break stage (stage 2), and the mature stage (stage 3). A total of 9 cDNA libraries (three independent biological replicates per developmental stage) were constructed and sequenced. This dataset captures the dynamic transcriptional reprogramming during fruit development, specifically highlighting the spatiotemporal expression patterns of genes involved in lipid metabolism, very-long-chain fatty acid (VLCFA) elongation, triterpenoid biosynthesis, and ABC transporter-mediated secretion. The provided RNA-seq data offer valuable insights into the molecular mechanisms of blueberry cuticular wax formation and serve as a fundamental resource for discovering key candidate genes for fruit quality improvement and genetic breeding.
2026-04-18 | GSE327820 | GEO
Project description:We report the application of high-throughput RNA sequencing for comparing the expression levels of the coding and long noncoding RNAs (lncRNAs) in leaf samples from a glossy mutant nwgl and its wild-type (WT) in cabbage. By obtaining over 163.35 Gb cleaned data generated from six libraries (on average, more than 26.48 Gb clean data for each sample replicate), we identified 1247 differential expressed genes (DEGs) and 148 differential expressed lncRNAs in nwgl leaves relative to WT. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed that the DEGs and cis-regulated target genes for differential expressed lncRNAs were significantly enriched in wax and lipid biosynthetic and/or metabolic processes. Our results provide the novel foundation to explore the complex molecular basis of cuticular wax biosynthesis.
2019-07-26 | GSE130405 | GEO
Project description:The goal of this study was to assay the extent of variation in chromatin organization between 3 ant castes (major and minor female workers and males) in one colony of Camponotus floridanus carpenter ant using ChIPseq. 45 samples total: 30 ChIP samples and 3 inputs for total histone H3, 7 histone H3 PTMs and RNA Pol II in major, minor, and male ants; CBP in major and minor ants; the major H3K27ac sample was replicated. 4 ChIP samples for H3 and H3K27ac in brains of majors and minors, and 2 inputs. 2 RNAseq samples for major and minor ants head+thorax; 4 RNAseq samples for brain (majors and minors with 2 replicates each).
2012-11-26 | E-GEOD-37523 | biostudies-arrayexpress