Project description:The proteomics study of thermophilic microorganisms in the sludge heat-treated at 75 degrees Celsius, to investigate the heat-resistant enzymes related to the hydrolysis of sludge organic matter.
Project description:In this study, centrifugal fractionation has been applied for fractionation of two biogas sludge samples to analyze proteins extracted from (i) crude fibers, (ii) suspended microorganisms, and (iii) secreted proteins in the supernatant using a gel-based approach followed by LC-MS/MS identification.
Project description:Heliophilum fasciatum is an anoxygenic photosynthetic bacteria isolated from rice soil. Previous work outlined the existence of five nitrogen-fixation-like gene clusters potentially involved in sulfur metabolism. In this study, cultures of H. fasciatum were grown with varying sulfate and thiosulfate concentrations, and the transcriptomes were generated using Illumina RNA-sequencing. Differential gene expression analysis was utilized to investigate sulfur-mediated regulation of these nitrogen-fixation-like systems and H. fasciatum genes.
Project description:The functional diversity of soil microbial communities was explored for a poplar plantation, which was treated solely with biogas slurry, or combined with biochar at different fertilization intensities over several years.
Project description:In this study, we used multiple meta-omic approaches to characterize the microbial community and the active metabolic pathways of a stable industrial biogas reactor operating at thermophilic temperatures (60°C) and elevated levels of free ammonia (367 mg NH3-N/L).