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Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock [S2]

ABSTRACT: Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock [S2]

PROVIDER: PRJNA541321 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9019610_1.fastq.gz	Fastqsanger.gz
	SRR9019610_2.fastq.gz	Fastqsanger.gz
	SRR9019611_1.fastq.gz	Fastqsanger.gz
	SRR9019611_2.fastq.gz	Fastqsanger.gz
	SRR9019612_1.fastq.gz	Fastqsanger.gz

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Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock [S2]

Project description:Heat shock (HS) causes dramatic and rapid changes in transcription of thousands of genes in metazoans. We have used insitu Hi-C in Drosophila S2 and human K562 cells to determine the effects of HS upon chromatin conformation. Our study shows that topologically associated domains (TADs), compartments and distal regulatory interactions remain unchanged upon HS.

2019-06-07 | GSE130776 | GEO

Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock

Project description:Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock

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Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock [K562]

2019-06-07 | GSE130758 | GEO

Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock [K562]

Project description:Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock [K562]

| PRJNA541309 | ENA

Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock.

Project description:Heat shock (HS) initiates rapid, extensive, and evolutionarily conserved changes in transcription that are accompanied by chromatin decondensation and nucleosome loss at HS loci. Here we have employed in situ Hi-C to determine how heat stress affects long-range chromatin conformation in human and Drosophila cells. We found that compartments and topologically associating domains (TADs) remain unchanged by an acute HS. Knockdown of Heat Shock Factor 1 (HSF1), the master transcriptional regulator of the HS response, identified HSF1-dependent genes and revealed that up-regulation is often mediated by distal HSF1 bound enhancers. HSF1-dependent genes were usually found in the same TAD as the nearest HSF1 binding site. Although most interactions between HSF1 binding sites and target promoters were established in the nonheat shock (NHS) condition, a subset increased contact frequency following HS. Integrating information about HSF1 binding strength, RNA polymerase abundance at the HSF1 bound sites (putative enhancers), and contact frequency with a target promoter accurately predicted which up-regulated genes were direct targets of HSF1 during HS. Our results suggest that the chromatin conformation necessary for a robust HS response is preestablished in NHS cells of diverse metazoan species.

| S-EPMC6765289 | biostudies-literature

Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-06-07 | GSE130778 | GEO