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RNAseq analysis of lacrimal glands isolated from wild type and Aire-/- mice at 5 weeks of age

ABSTRACT: RNAseq analysis of lacrimal glands isolated from wild type and Aire-/- mice at 5 weeks of age

PROVIDER: PRJNA542600 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9047180.fastq.gz	Fastqsanger.gz
	SRR9047181.fastq.gz	Fastqsanger.gz
	SRR9047182.fastq.gz	Fastqsanger.gz
	SRR9047183.fastq.gz	Fastqsanger.gz
	SRR9047184.fastq.gz	Fastqsanger.gz

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RNAseq analysis of lacrimal glands isolated from wild type and Aire-/- mice at 5 weeks of age

Project description:We used freshly isolated lacrimal glands isolated from wild type and Aire-/- mice on a Balbc background. Mice were 5 weeks of age, with an n=4 for wild type and n=3 for Aire-deficient.

2019-05-14 | GSE131073 | GEO

RNA-Sequencing of Regenerating Lacrimal Glands

Project description:The purpose of the present studies was to use CyTOF and RNA-Seq technologies to identify cells and genes involved in lacrimal gland repair that could be targeted to treat diseases of lacrimal gland dysfunction. Lacrimal glands of female BALB/c mice were experimentally injured by intra-glandular injection of interleukin 1 alpha (IL-1α). The lacrimal glands were harvested at various time points following injury (1 to 14 days) and used to either prepare single cell suspensions for CyTOF immuno-phenotyping analyses or to extract RNA for gene expression studies using RNA-Seq. CyTOF immuno-phenotyping identified monocytes and neutrophils as the major infiltrating populations 1 and 2 days post injury. Clustering of significantly differentially expressed genes identified 13 distinct molecular signatures: 3 associated with immune/inflammatory processes included genes up-regulated at days 1-2 and 3 associated with reparative processes with genes up-regulated primarily between days 4 and 5. Finally, clustering identified 65 genes which were specifically up-regulated 2 days post injury which was enriched for muscle specific genes. The expression of select muscle-related proteins was confirmed by immunohistochemistry which identified a subset of cells expressing these proteins. Double staining experiments showed that these cells are distinct from the myoepithelial cells. We conclude that experimentally induced injury to the lacrimal gland leads to massive infiltration by neutrophils and monocytes which resolved after 3 days. RNAseq and immunohistochemistry identified a group of cells, other than myoepithelial cells, that express muscle-related proteins that could play an important role in lacrimal gland repair.

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Transcription profiling of pancreata, submandibular, and lacrimal glands from NOD-scid and C57Bl/6-scid mice to study the gene expression patterns of nonobese diabetic mice.

Project description:Prior to the onset of autoimmune destruction, type 1 diabetic patients and an animal model thereof, the nonobese diabetic (NOD) mouse, show morphological and functional abnormalities in target organs, which may act as inciting events for leukocyte infiltration. To better understand these abnormalities, but without the complications associated with inflammatory infiltrates, we examined genes expressed in autoimmune target tissues (pancreas, submandibular glands, and lacrimal glands) of NOD/scid mice and of autoimmune-resistant C57BL6/scid mice. Experiment Overall Design: Pancreata (6 weeks old mice), submandibular (9 and 15 weeks), and lacrimal glands (15 weeks) from individual NOD-scid and B6-scid mice were isolated for RNA extraction and hybridization on Affymetrix microarrays.

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Project description:NOD mice were injected once a week with LTBR-Ig to block the LTBR-pathway, or with control monoclonal antibody MOPC from age 8 to 16 weeks old. Extraorbital lacrimal glands or submaxillary glands were dissected and total mRNA prepared. Each sample was either the combined lacrimals (2) from each mouse or individual salivary glands. There were 4 mice in each treatment group. Total mRNA was isolated and the quality was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). Reverse transcription to prepare cDNA was performed using Invitrogen M-MLV system. The purpose was to determine changes in gene expression in glands due to blockade of the LTBR-pathway. Differential Gene Expression in NOD mouse lacrimal and salivary glands after LTBR-Ig treatment

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Female BALB/c Mouse Testosterone-Treated Submandibular/Lacrimal/Meibomian Glands

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Ectopic lymphoid structures in the aged lacrimal glands

Project description:Ectopic lymphoid structures in the aged lacrimal glands

| PRJNA931932 | ENA

Alterations in gene expression in lacrimal and salivary glands of male NOD mice due to LTBR-Ig treatment relative to control antibody

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