Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human Pluripotent Stem Cell-Derived Kidney Organoids for Modeling Epithelial Transport and Injury

ABSTRACT: Human Pluripotent Stem Cell-Derived Kidney Organoids for Modeling Epithelial Transport and Injury

PROVIDER: PRJNA542638 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9047573_1.fastq.gz	Fastqsanger.gz
	SRR9047573_2.fastq.gz	Fastqsanger.gz
	SRR9047574_1.fastq.gz	Fastqsanger.gz
	SRR9047574_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Human Pluripotent Stem Cell-Derived Kidney Organoids for Modeling Epithelial Transport and Injury

Project description:Maximizing the potential of human kidney organoids for drug testing, regenerative medicine and to model development and disease requires addressing cell immaturity, the lack of a branching collecting system and the off target cell types. Here we establish methods to independently generate the two kidney progenitor cell populations – metanephric mesenchyme and ureteric bud. Combining these two progenitor cell types results in organoids with an improved branched collecting system. We also identified the hormones aldosterone and arginine vasopressin as critical to promote maturation of collecting duct cell types. The resulting organoids contain the full range of epithelial cells in the nephron, including principal and intercalated cells. By scRNA-seq, we demonstrate superior proximal tubule maturation and reduced off-target cell populations using this protocol.

2020-12-15 | GSE131086 | GEO

Modeling oxidative injury response in human kidney organoids

Project description:Modeling oxidative injury response in human kidney organoids

| PRJNA755987 | ENA

Modeling heart failure by induced pluripotent stem cell-derived organoids.

Project description:Modeling heart failure by induced pluripotent stem cell-derived organoids.

| PRJNA1201861 | ENA

Modeling oxidative injury response in human kidney organoids

Project description:Analysis of human kidney organoids treated with hemin for 48 hrs, to induce reactive oxygen injury.

2021-12-31 | GSE182350 | GEO

Human Pluripotent Stem Cell-Derived Alveolar Organoids for Gene-editing and Lung Adenocarcinomas Modeling

Project description:Human pluripotent stem cell (hPSC)-derived alveolar organoids have emerged as valuable tools for studying lung development, modeling pulmonary diseases, and drug discovery, though their application has been hindered by laborious differentiation protocols and technical complexity. Here, we present an hPSC_x0002_derived alveolar organoid (hALO) system with exceptional long-term expandability (>30 passages), efficient cryopreservation resilience, and streamlined production achieved through earlier 3D culture initiation and elimination of cell sorting requirements. Transcriptomic analysis across passages confirmed hALOs contain alveolar progenitors and AT2 lineages, recapitulating pseudoglandular-to-canalicular development while partially maintaining adult AT2 immune-related functions. The system permits alveolar epithelial differentiation via pharmacological modulation of WNT/YAP signaling or through orthotopic transplantation, while multiplex genetic engineering enables programmable disease modeling and adenocarcinoma pathogenesis studies. These versatile capabilities establish hALOs as a robust dual-phase platform for mechanistic investigation of lung epithelial biology and disease modeling across in vitro and in vivo environments.

2025-09-27 | GSE308817 | GEO

A developmentally-inspired hypoxia condition promotes kidney organoids differentiation from human pluripotent stem cells

Project description:Kidney organoids derived from human pluripotent stem cells (hPSCs) lack a contiguous network of collecting ducts, which limits their utility in modeling kidney development and disease. Here, we report the generation of kidney organoids containing ureteric bud (UB)-derived collecting ducts connected to metanephric mesenchyme (MM)-derived nephrons using a developmentally-inspired hypoxic differentiation condition. Hypoxia promotes a reiterative process of branching morphogenesis and nephron induction through reciprocal interactions between co-induced MM and UB, which lead to a higher-order kidney organogenesis in vitro. The resulting kidney organoids demonstrate greater maturity, as indicated by higher levels of functional markers and a more realistic micro-anatomy. Additionally, these hypoxic-enhanced kidney organoids show a great potential as in vitro models for renal cystic diseases, as they efficiently generate cystic formations and display high sensitivity to drugs. This hypoxia approach may open new avenues for an enhanced understanding of kidney development and diseases.

2025-01-05 | GSE252639 | GEO

Cardiac Organoids for Modeling Hypertrophic Cardiomyopathy

Project description:Cardiac Organoids for Modeling Hypertrophic Cardiomyopathy

| PRJNA1094569 | ENA

Modeling PD in midbrain-like organoids

Project description:Modeling PD in midbrain-like organoids

| PRJNA525884 | ENA

Proteome analysis of brainstem organoids from human pluripotent stem cells

Project description:Brain organoids derived from human pluripotent stem cells recapitulate the course of human brain development. We applied proteomic analysis to human brainstem organoids which were developed by a novel method.

2020-05-01 | PXD016807 | JPOST Repository

Human Pluripotent Stem Cell-Derived Alveolar Organoids for Gene-editing and Lung Adenocarcinomas Modeling

Project description:Human Pluripotent Stem Cell-Derived Alveolar Organoids for Gene-editing and Lung Adenocarcinomas Modeling

| PRJNA1332757 | ENA