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Pseudohyphal differentiation in Komagataella phaffii: investigating the FLO gene family

ABSTRACT: Pseudohyphal differentiation in Komagataella phaffii: investigating the FLO gene family

PROVIDER: PRJNA542662 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9048190.fastq.gz	Fastqsanger.gz
	SRR9048191.fastq.gz	Fastqsanger.gz
	SRR9048192.fastq.gz	Fastqsanger.gz
	SRR9048183.fastq.gz	Fastqsanger.gz
	SRR9048193.fastq.gz	Fastqsanger.gz

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Pseudohyphal differentiation in Komagataella phaffii: investigating the FLO gene family

Project description:The protein production host Komagataella phaffii has possess the ability to differentiate into pseudohyphal form when cultivated at slow growth rates (µ=0.05 h-1) in glucose-limited chemostats. In this study, we investigated the K. phaffii FLO gene family in the context of pseudohyphae formation. Transcriptional analysis helped us identify 3 possible responsible genes, FLO11, FLO400 and FLO5-1, all of which are under control of Flo8, a transcription factor whose disruption prevents pseudohyphae formation. Knocking out FLO11 revealed that this is not the sole protein responsible for this phenotype. Strikingly, the expression of FLO400 and FLO5-1 was negatively correlated with pseudohyphae formation, and shown to be under epigenetic control by FAIRE-Seq analysis. Knock outs of these two genes completely inhibited the appearance of pseudohyphal cells and prevented the expression of FLO11. Even though the mechanism is unclear at present, we propose that in K. phaffii Flo400 and/or Flo5-1 act as upstream signals that lead to the induction of FLO11 expression upon severe glucose limitation in chemostats at slow growth rate, and that the expression of FLO400 and FLO5-1 is controlled by epigenetic silencing, which acts independently from the general activation of FLO gene expression by the transcriptional regulator Flo8.

2020-08-19 | GSE131106 | GEO

Pseudohyphal differentiation in Komagataella phaffii: investigating the FLO gene family (FAIRE-seq)

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Pseudohyphal differentiation in Komagataella phaffii: investigating the FLO gene family (FAIRE-seq)

Project description:Pseudohyphal differentiation in Komagataella phaffii: investigating the FLO gene family (FAIRE-seq)

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Pseudohyphal differentiation in Komagataella phaffii: investigating the FLO gene family.

Project description:Many yeasts differentiate into multicellular phenotypes in adverse environmental conditions. Here, we investigate pseudohyphal growth in Komagataella phaffii and the involvement of the flocculin (FLO) gene family in its regulation. The K. phaffii FLO family consists of 13 members, and the conditions inducing pseudohyphal growth are different from Saccharomyces cerevisiae. So far, this phenotype was only observed when K. phaffii was cultivated at slow growth rates in glucose-limited chemostats, but not upon nitrogen starvation or the presence of fusel alcohols. Transcriptional analysis identified that FLO11, FLO400 and FLO5-1 are involved in the phenotype, all being controlled by the transcriptional regulator Flo8. The three genes exhibit a complex mechanism of expression and repression during transition from yeast to pseudohyphal form. Unlike in S. cerevisiae, deletion of FLO11 does not completely prevent the phenotype. In contrast, deletion of FLO400 or FLO5-1 prevents pseudohyphae formation, and hampers FLO11 expression. FAIRE-Seq data shows that the expression and repression of FLO400 and FLO5-1 are correlated to open or closed chromatin regions upstream of these genes, respectively. Our findings indicate that K. phaffii Flo400 and/or Flo5-1 act as upstream signals that lead to the induction of FLO11 upon glucose limitation in chemostats at slow growth and chromatin modulation is involved in the regulation of their expression.

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