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Pancreatic Epithelial Single Cell RNAseq in OGT-deficient cells

ABSTRACT: Pancreatic Epithelial Single Cell RNAseq in OGT-deficient cells

PROVIDER: PRJNA542702 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9050451.fastq.gz	Fastqsanger.gz
	SRR9050452.fastq.gz	Fastqsanger.gz
	SRR9050453.fastq.gz	Fastqsanger.gz
	SRR9050454.fastq.gz	Fastqsanger.gz

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Pancreatic Epithelial Single Cell RNAseq in OGT-deficient cells

Project description:Single Cell RNAseq was performed to identify cell types and upstream regulators in pancreatic epithelial cells with or without OGT.

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Transcriptome analysis of OGT-sufficient and OGT-deficient regulatory T (Treg) cells

Project description:To gain comprehensive insight into the OGT-dependent transcriptional program in Treg cells, we performed RNA-sequencing of isolated YFP+ Treg cells from Foxp3YFP-Cre/wtOgtwt/fl and healthy Foxp3YFP-Cre/wtOgtfl/fl females to avoid secondary changes in gene expression caused by inflammation. We were able to identify 269 differentially expressed genes including 154 downregulated and 115 upregulated with p values less than 0.01, OGT-deficient Treg cells had impaired suppressive function and attenuated IL2/STAT5 signaling pathway.

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Genome-wide changes in OGT-deficient mouse liver tissue

Project description:Over a billion people suffer from chronic liver diseases worldwide, which often leads to fibrosis and then cirrhosis. Treatments for fibrosis remain experimental, in part because no unifying mechanism has been identified that initiates liver fibrosis. O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) plays a pro-survival role under stress in many tissues. Here we report that OGT protects against hepatocyte necroptosis and initiation of liver fibrosis. Decreased O-GlcNAc levels were seen in patients with alcoholic liver cirrhosis and in mice with ethanol-induced liver injury. Liver-specific O-GlcNAc transferase (OGT) knockout (OGT-LKO) mice progressed to liver fibrosis at 10 weeks of age. OGT-deficient hepatocytes underwent necroptosis. These findings identify OGT as a key suppressor of hepatocyte necroptosis and OGT-LKO mice may serve as an effective spontaneous genetic model of liver fibrosis.

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RNA-seq analysis of Ogt regulated genes in smooth muscle cells (VSMCs)

Project description:To investigate the roles of Ogt in smooth muscle cells (SMCs), we crossed Ogt floxed mice and SM22-CreER mice to generate inducible SMC Ogt specific knockout mice. Aorta tissues from these mice were isolated and RNA were extracted and performed RNAseq analysis.

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OGT, H2B S112GlcNAc, TET2 ChIP-seq results

Project description:TET2 directly interacts with OGT, which is important for the chromatin association of OGT in vivo. Although this specific interaction does not regulate the enzymatic activity of TET2, it facilitates OGT-dependent histone O-GlcNAcylation. Moreover, OGT associates with TET2 at transcription starting sites (TSS). Down-regulation of TET2 reduces the amount of H2B S112 GlcNAc marks in vivo, which are associated with gene transcription regulation. We found that OGT interacts with TET2 tightly. Using ChIP-seq with specific antibodies, we tested the co-localization of TET2 and OGT in genome level.

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