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Small RNAs during seed development in a small-seeded chickpea

ABSTRACT: Small RNAs during seed development in a small-seeded chickpea

PROVIDER: PRJNA543802 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9090780.fastq.gz	Fastqsanger.gz
	SRR9090790.fastq.gz	Fastqsanger.gz
	SRR9090781.fastq.gz	Fastqsanger.gz
	SRR9090791.fastq.gz	Fastqsanger.gz
	SRR9090782.fastq.gz	Fastqsanger.gz

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Small RNAs during seed development in a small-seeded chickpea

Project description:In this study, we sequenced small RNAs at seven successive stages of seed development and leaf tissue in a small-seeded chickpea cultivar (Himchana 1) using Illumina platform. More than 500 million reads were generated in all the samples combined together with an average of 34 million reads in each sample. Data obtained in FASTQ files were pre-processed and unique reads representing small RNAs were identified at different stages of seed development.

2020-12-31 | GSE131431 | GEO

Small RNAs during seed development in large-seeded chickpea

Project description:In this study, we sequenced small RNAs at seven successive stages of seed development and leaf tissue in a large-seeded chickpea cultivar (JGK 3) using Illumina platform. More than 500 million reads were generated in all the samples combined together with an average of 34 million reads in each sample. Data obtained in FASTQ files were pre-processed and unique reads representing small RNAs were identified at different stages of seed development.

2020-07-13 | GSE131424 | GEO

Small RNAs during seed development in large-seeded chickpea

Project description:Small RNAs during seed development in large-seeded chickpea

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DNA methylation during seed development in small-seeded chickpea

Project description:In this study, we performed bisulphite of two stages of seed development in a small-seeded chickpea cultivar (Himchana 1) using Illumina platform. Paired-end reads were generated from 5 libraries. Data obtained in FASTQ files were pre-processed to remove adapters and low-quality reads. We identified methylation level at each cytosine residue covered in sequencing and differentially methylated regions (DMRs) between stages of seed development.

2020-07-13 | GSE131669 | GEO

DNA methylation during seed development in small-seeded chickpea

Project description:DNA methylation during seed development in small-seeded chickpea

| PRJNA544333 | ENA

DNA methylation during seed development in large-seeded chickpea

Project description:In this study, we performed bisulphite of five stages of seed development in a large-seeded chickpea cultivar (JGK 3) using Illumina platform. Paired-end reads were generated from 11 libraries. Data obtained in FASTQ files were pre-processed to remove adapters and low-quality reads. We identified methylation level at each cytosine residue covered in sequencing and differentially methylated regions (DMRs) between stages of seed development.

2020-07-13 | GSE131665 | GEO

Transcriptome dynamics during seed development in chickpea (Himchana)

Project description:In this study, we aim to present a global view of transcriptome dynamics during seed development in a small-seeded chickpea (genotype Himchana 1). We generated about 1.5 billion high-quality reads from 24 libraries (leaf and seven seed developmental stages in three biological replicates) using Illumina high-throughput sequencing platform. We mapped the reads to the kabuli chickpea genome for estimation of their transcript abundance in different tissue samples. The transcriptome dynamics was studied by differential gene expression analyses between different samples/stages.

2017-12-31 | GSE79720 | GEO

DNA methylation during seed development in large-seeded chickpea

Project description:DNA methylation during seed development in large-seeded chickpea

| PRJNA544329 | ENA

Transcriptome dynamics during seed development in chickpea (JGK3)

Project description:In this study, we aim to present a global view of transcriptome dynamics during seed development in a large-seeded chickpea (genotype JGK3). We generated about 1.5 billion high-quality reads from 24 libraries (leaf and seven seed developmental stages in three biological replicates) using Illumina high-throughput sequencing platform. We mapped the reads to the kabuli chickpea genome for estimation of their transcript abundance in different tissue samples. The transcriptome dynamics was studied by differential gene expression analyses between different samples/stages.

2017-12-31 | GSE79719 | GEO

Small RNA Solexa sequencing of chickpea (Cicer arietinum)

Project description:The total RNA were extracted from pooled tissues of leaves and flowers from several plants of chickpea (Cicer arietinum) using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Then small RNAs ranging in 18–30 nucleotides were size fractionated electrophoretically, isolated from the gel, ligated with the 5′ and 3′ RNA adapters. The ligated product was reverse transcribed and subsequently amplified using 10–12 PCR cycles. The purified PCR product was sequenced using Illumina Genome Analyzer II. The qualified reads were used to predict microRNAs and phased small interfering RNAs from chickpea. Identification of microRNAs and phased small inferfering RNAs in chickpea (Cicer arietinum) by analyzing small RNA sequencing profiles of leaves and flowers using Illumina GAII.

2015-05-08 | E-GEOD-62216 | biostudies-arrayexpress

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