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Identification of monocyte-like precursors of granulocytes as a mechanism for accumulation of PMN-MDSC in cancer

ABSTRACT: Identification of monocyte-like precursors of granulocytes as a mechanism for accumulation of PMN-MDSC in cancer

PROVIDER: PRJNA544005 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9099370.fastq.gz	Fastqsanger.gz
	SRR9099371.fastq.gz	Fastqsanger.gz
	SRR9099366.fastq.gz	Fastqsanger.gz
	SRR9099367.fastq.gz	Fastqsanger.gz
	SRR9099368.fastq.gz	Fastqsanger.gz

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Similar Datasets

Identification of monocyte-like precursors of granulocytes as a mechanism for accumulation of PMN-MDSC in cancer

Project description:Polymorphonuclear myeloid-derived suppressor cells (PMN-MDSC) inhibit immune responses in cancer, limit the effects of therapies and promote tumor cell metastasis. Here, we have identified a new precursor that differentiates into granulocytes in vitro and in vivo yet belong to the monocytic lineage. We have termed these cells monocyte-like precursors of granulocytes (MLPG). Under steady state conditions MLPG were absent in the spleen and barely detectable in the bone marrow (BM). In contrast, these cells significantly expanded in tumor-bearing mice. Selective depletion of monocytic cells had no effect on the number of granulocytes present in naïve mice but decreased the population of PMN-MDSC in tumor-bearing mice by 50%. The expansion of MLPG was found to be controlled by the down-regulation of the protein Rb1 and not the IRF8 transcription factor that is known to regulate the expansion of PMN-MDSC from classical granulocytes precursors. In cancer patients, putative MLPG were found within the population of CD15 CD14+HLA-DR-/lo M-MDSC. CXCR1+CD15 CD14+HLA-DR-/lo cells lacked immune suppressive activity but had potential to differentiate to neutrophils in contrast to monocytes and CXCR1- suppressive M-MDSC. These findings describe a mechanism of abnormal myelopoiesis in cancer and suggest potential new approaches for selective targeting of MDSC.

2019-05-30 | GSE131552 | GEO

Identification of monocyte-like precursors of granulocytes as a mechanism for accumulation of PMN-MDSC in cancer [microarray]

2019-05-30 | GSE131516 | GEO

Identification of monocyte-like precursors of granulocytes as a mechanism for accumulation of PMN-MDSC in cancer [microarray]

Project description:Identification of monocyte-like precursors of granulocytes as a mechanism for accumulation of PMN-MDSC in cancer [microarray]

| PRJNA543867 | ENA

Transcriptome comparsion of WT and CD300ld KO PMN-MDSC

Project description:Polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC), also named pathologically activated neutrophil, is a critical component of tumor microenvironment (TME), playing crucial roles in tumor progression and therapy resistance. CD300ld is specifically expressed in normal neutrophils and is upregulated in PMN-MDSCs upon tumor bearing. CD300ld knockout (KO) inhibits the development of multiple tumor types in a PMN-MDSC-dependent manner. Here, we compared the transcriptome of PMN-MDSCs from WT mice and CD300ld KO mice.

2023-06-26 | GSE199602 | GEO

Whole genome expression profile of human decidual PMN-MDSC in normal pregnancy and unexplained recurrent pregnancy loss

Project description:Polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC) accumulate in maternal-fetal interface during pregnancy and play a role in maintenance of immune tolerance. Decreased PMN-MDSC is associated with pregnancy complications such as unexplained recurrent pregnancy loss (URPL). Whether PMN-MDSC function is different between normal pregnancy (NP) and URPL remains unexplored. Here we performed whole genome expression profile of 3 decidual PMN-MDSC from normal early pregnancy and 3 decidual PMN-MDSC from URPL. Total RNA were extracted. Cy5-labeled aRNA was hybridized and scanned on a G2505C Agilent Microarray Scanner with Agilent 0.1 XDR software. The gene expression pattern of the PMN-MDSC was significantly different between the NP group and the URPL group.

2020-06-30 | GSE139180 | GEO

Identification of monocyte-like precursors of granulocytes in cancer as a mechanism for accumulation of PMN-MDSCs.

Project description: Not available

| S-EPMC6719429 | biostudies-literature

Effect of Pseudomonas aeruginosa (WT and dFliC) infection on PMN- and M-MDSC

Project description:Pseudomonas aeruginosa (P.a.) is a pathogenic opportunistic bacterium, classified as a priority by the WHO for the research of new treatments. As this bacterium is harmful trough the inflammation and tissue damage it causes, we investigated the role of Myeloid Derived Suppressor Cells (MDSC) in P.a. infections and their potential as a therapeutic tool. Using both ‘classically’ obtained MDSC (through mice bone-marrow differentiation), and a new procedure developped here (using the ER-Hoxb8 hematopoietic cell line), we observed that after administering intra-nasally a lethal dose of P.a (PAO1), intra-pulmonary transfer of MDSC, in both prophylactic and therapeutic protocols, markedly improves survival of P.a infected animals. Mechanistically, with a sub-lethal dose of P.a, we observed that MDSC transfer modulated lung tissue injury, down-regulated inflammatory responses and elicited lung repair. We further showed that WT-PAO1 and MDSC (and their subtypes PMN-MDSC and M-MCSF) could interact directly in vitro and in vivo, and that both PMN- and M-MDSC gene expression (assessed through RNA sequencing) was modulated after in vitro P.a. infection, and that WT-PAO1 (but not dFlic-PAO1) infection led to inhibition of T cell proliferation and promoted epithelial cell wound healing. Furthermore, we showed that the transcription factor Nr4A1 was up-regulated in both PMN- and M-MDSC- infected cells and may be an important mediator in the process. Altogether, we highlight a potential beneficial role of MDSC in P.a. infection responses and suggest that the unique properties of MDSC make them attractive potential new therapeutic tools for patients with acute or chronic inflammatory diseases.

2025-04-11 | GSE287469 | GEO

NGS analysis of Myeloid-Derived Suppressor Cells (MDSC) and Granulocytes isolated directly after major surgery

Project description:The intention of this experiment was to detect differences in gene expression of acutely and transiently occurring Myeloid-Derived Suppressor Cells (MDSC) and Granulocytes directly after a strong surgical stimulus.

2025-08-29 | E-MTAB-14974 | biostudies-arrayexpress

Transcriptome comparsion of WT and CD300ld KO PMN-MDSC

Project description:Transcriptome comparsion of WT and CD300ld KO PMN-MDSC

| PRJNA820787 | ENA

Dabrafenib alters MDSC differentiation and function by activation of GCN2

Project description:The BRAF inhibitor dabrafenib has been reported to activate the integrated stress response (ISR) kinase GCN2, and the therapeutic effect has been partially attributed to GCN2 activation. Since ISR signaling is a key component of myeloid-derived suppressor cell (MDSC) development and function, we measured the effect of dabrafenib on MDSC differentiation and suppressive activity. Our data showed that dabrafenib attenuated MDSC ability to suppress T cell activity, which was associated with a GCN2-dependent block of the transition from monocytic progenitor to polymorphonuclear (PMN)-MDSCs and proliferative arrest resulting in PMN-MDSC loss. Transcriptional profiling revealed that dabrafenib-driven GCN2 activation altered metabolic features in MDSCs enhancing oxidative respiration, and attenuated transcriptional programs required for PMN development. Thus, ourdata reveals transcriptional networks that govern MDSC developmental programs, and the impact of GCN2 stress signaling on the innate immune landscape in tumors, providing novel insight into potentially beneficial off target effects of dabrafenib.

2024-03-20 | GSE239496 | GEO

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