Project description:Suberoylanilide hydroxamic acid (SAHA) and valproic acid (VPA) are both histone deacetylases inhibitor (HDACi), and are able to attenuate the activation of hepatic stelllate cells. To explore the underlying molecular mechanisms, we performed gene expression profile analyses of human hepatic stellate cell line LX2 treated with SAHA or VPA for 24 hours. Duplicate experiments were performed: Untreated LX2, SAHA treated LX2 and VPA treated LX2.
Project description:Suberoylanilide hydroxamic acid (SAHA) and valproic acid (VPA ) are both histone deacetylases inhibitor (HDACi), and are able to attenuate the activation of hepatic stelllate cells. To explore the underlying molecular mechanisms, we performed miRNA expression profile analyses of human hepatic stellate cell line LX2 treated with SAHA or VPA for 24 hours. Duplicate experiments were performed: Untreated LX2, SAHA treated LX2 and VPA treated LX2.
Project description:Background CD44 is a transmembrane protein, which plays an essential role for transducing extracellular stimuli into intracellular signaling cascades especially in cancer cells. Recent studies have shown that CD44 may contribute to metabolic regulation. However, whether and how CD44 affects adipocyte differentiation in white adipose tissue (WAT) remain unclear. Wild type (WT) and CD44 knockout (KO) mice were fed with high-fat diet to induce obesity. eWAT from mice were then harvested for RNA-seq
Project description:To investigate whether metoprolol treatment could affect the transcriptome and translatome of killing supernatant cultured human monocytes