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Single cell RNA-seq analysis reveals the crucial role of Collagen triple helix repeat containing 1 (Cthrc1) cardiac fibroblasts for ventricular remodeling after myocardial infarction

ABSTRACT: Single cell RNA-seq analysis reveals the crucial role of Collagen triple helix repeat containing 1 (Cthrc1) cardiac fibroblasts for ventricular remodeling after myocardial infarction

PROVIDER: PRJNA546019 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10349516.fastq.gz	Fastqsanger.gz
	SRR10349517.fastq.gz	Fastqsanger.gz
	SRR10349518.fastq.gz	Fastqsanger.gz
	SRR10349519.fastq.gz	Fastqsanger.gz
	SRR10349520.fastq.gz	Fastqsanger.gz

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Single cell RNA-seq analysis reveals the crucial role of Collagen triple helix repeat containing 1 (Cthrc1) cardiac fibroblasts for ventricular remodeling after myocardial infarction [RNA-seq]

Project description:Cardiac fibroblasts have a central role during the ventricular remodeling process associated to different types of cardiac injury. Recent studies have shown that fibroblasts do not respond homogeneously to heart damage, suggesting that the adult myocardium may contain specialized fibroblast subgroups with specific functions. Due to the limited set of bona fide fibroblast markers, a proper characterization of fibroblast population dynamics in response to cardiac damage is still missing. Using single cell RNA-seq we have identified and characterized a fibroblast subpopulation that emerges in response to myocardial infarction (MI) in a murine model. These activated fibroblasts exhibit a clear pro-fibrotic signature, express high levels of the hormone CTHRC1 and of the immunomodulatory co-receptor CD200, and localize to the wounded myocardium. Combining epigenomic profiling with functional assays, we unveil important candidate regulators mediating their response to cardiac damage. We show that the absence of CTHRC1, a top marker of this activated fibroblast subpopulation, results in pronounced lethality due to ventricular rupture within the first week post-myocardial infarction. Finally, we find evidence for the existence of similar mechanisms in a pig pre-clinical model of MI and establish a positive correlation between Cthrc1 levels and cardiac function after MI.

2020-09-29 | GSE132143 | GEO

Single cell RNA-seq analysis reveals the crucial role of Collagen triple helix repeat containing 1 (Cthrc1) cardiac fibroblasts for ventricular remodeling after myocardial infarction [scRNA-seq]

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Single cell RNA-seq analysis reveals the crucial role of Collagen triple helix repeat containing 1 (Cthrc1) cardiac fibroblasts for ventricular remodeling after myocardial infarction [ATAC-seq]

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Single cell RNA-seq analysis reveals the crucial role of Collagen triple helix repeat containing 1 (Cthrc1) cardiac fibroblasts for ventricular remodeling after myocardial infarction [ATAC-seq]

Project description:Single cell RNA-seq analysis reveals the crucial role of Collagen triple helix repeat containing 1 (Cthrc1) cardiac fibroblasts for ventricular remodeling after myocardial infarction [ATAC-seq]

| PRJNA546022 | ENA

Single cell RNA-seq analysis reveals the crucial role of Collagen triple helix repeat containing 1 (Cthrc1) cardiac fibroblasts for ventricular remodeling after myocardial infarction [RNA-seq]

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Single cell RNA-seq analysis reveals the crucial role of Collagen triple helix repeat containing 1 (Cthrc1) cardiac fibroblasts for ventricular remodeling after myocardial infarction [scRNA-seq]

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Effect of CTHRC1 knockdown on gene expression of HCCLM3 cells

Project description:Collagen triple helix repeat containing 1 (CTHRC1) has been found to be up-regulated in many human solid tumors. In this study, we investigated the changes of gene expression by comparing CTHRC1-siRNA and Scramble-siRNA control in hepatocellular carcinoma cell line HCCLM3, which has high expression levels of CTHRC1. Differential gene expression was assessed by Affymetrix microarray experiments for 2 samples: CTHRC1-siRNA-treated HCCLM3 cells and Scramble-siRNA-treated HCCLM3 cells.

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Effect of CTHRC1 knockdown on gene expression of HCCLM3 cells

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Transcriptional profiling of wounds in WT mice and Cthrc1-/- mice

Project description:Collagen triple helix repeat containing 1 (CTHRC1) has been reported to be associated with tissue repair in response to injury. To investigate the role of CTHRC1 in the skin wound repair, we used next-generation sequencing (NGS) to clarify the changes of transcriptome in the mice skin wounds after Cthrc1 deletion.

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Single cell RNA-seq analysis reveals the crucial role of Collagen triple helix repeat containing 1 (Cthrc1) cardiac fibroblasts for ventricular remodeling after myocardial infarction

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-09-29 | GSE132146 | GEO