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Identification of potassium phosphite responsive miRNAs and their targets in potato

ABSTRACT: Identification of potassium phosphite responsive miRNAs and their targets in potato

PROVIDER: PRJNA546567 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9203810.fastq.gz	Fastqsanger.gz
	SRR9203811.fastq.gz	Fastqsanger.gz
	SRR9203812.fastq.gz	Fastqsanger.gz
	SRR9203813.fastq.gz	Fastqsanger.gz
	SRR9203808.fastq.gz	Fastqsanger.gz

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Project description:Phytophthora root rot caused by the hemibiotrophic oomycete, Phytophthora cinnamomi is a major biotic hindrance in meeting the ever-increasing demand for avocados. In addition, the pathogen is a global menace to agriculture, horticulture and forestry. Phosphite trunk injections/foliar sprays remains the most effectual chemical management strategy followed in commercial avocado orchards to control the pathogen. Phosphite is known to counter the pathogen both directly and indirectly through fortification of host defense. However, phosphite’s direct mode of action is still not understood completely. This study identified a P. cinnamomi isolate GKB4 sensitive to phosphite (EC50 of 27.9 μg/mL) and investigated the phosphite’s direct impact on this isolate through label-free quantitative SWATH-MS. Proteomics data analysis of untreated vs phosphite-treated samples revealed that the xenobiotic affects the pathogen’s growth by targeting the oxidoreductases whose abundance is significantly reduced. Further, perturbations in the energy metabolism and membrane/transmembrane proteins and transporters, and oxidative stress contribute to growth inhibition. The current study also identified increased putrescine biosynthesis, a polyamine, that when present at non-optimal concentrations could be cytostatic/cytotoxic. The differential expression of enzymes involved in the biosynthesis of secondary metabolites and the intermediates/precursors involved in their biosynthesis is an interesting finding that needs further investigation to ascertain their role in phosphite induced stress. The pathogen’s counteraction in the form of upregulation of alternate bioenergetics pathways (amino acid catabolism and -oxidation of fatty acids), mitochondrial translation and translocation machinery, peroxisomal proteins, and antioxidants are apparently ineffective in arresting the phosphite’s growth-inhibitory effects. This research furthers our limited understanding of the direct in vitro effects of phosphite on P. cinnamomi and has identified potential candidates for molecular functional investigation.

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Identification of potassium phosphite responsive miRNAs and their targets in potato

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