Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

NF90 Modulates Processing of a Subset of Human Pri-miRNAs

ABSTRACT: NF90 Modulates Processing of a Subset of Human Pri-miRNAs

PROVIDER: PRJNA547631 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9212450.fastq.gz	Fastqsanger.gz
	SRR9212460.fastq.gz	Fastqsanger.gz
	SRR9212530.fastq.gz	Fastqsanger.gz
	SRR9212451.fastq.gz	Fastqsanger.gz
	SRR9212461.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

NF90 Modulates Processing of a Subset of Human Pri-miRNAs

Project description:This SuperSeries is composed of the SubSeries listed below. MicroRNAs are predicted to regulate the expression of more than 60% of mammalian genes and play fundamental roles in most biological processes. Deregulation of miRNA expression is a hallmark of most cancers and further investigation of mechanisms controlling miRNA biogenesis is needed. The dsRNA-binding protein, NF90 has been shown to act as a competitor of Microprocessor for a limited number of pri-miRNAs. Here, we show that NF90 has a more widespread effect on pri-miRNA biogenesis than previously thought. Genome-wide approaches revealed that NF90 is associated with the stem region of 38 pri-miRNAs, in a manner that is largely exclusive of Microprocessor. Following loss of NF90, 25 NF90-bound pri-miRNAs showed increased abundance of mature miRNA products. NF90-targeted pri-miRNAs are highly stable, having a lower free energy and fewer mismatches compared to all pri-miRNAs. Mutations leading to less stable structures reduced NF90 binding while increasing pri-miRNA stability led to ac quisition of NF90 association, as determined by RNA EMSA. NF90-bound and modulated pri-miRNAs are embedded in introns of host genes and expression of several is concomitantly modulated, including an oncogene implicated in metastasis of hepatocellular carcinoma, TIAM2. These data suggest that NF90 controls the processing of a subset of highly stable, intronic miRNAs.

2020-05-01 | GSE132341 | GEO

NF90 modulates processing of a subset of human pri-miRNAs.

Project description:MicroRNAs (miRNAs) are predicted to regulate the expression of >60% of mammalian genes and play fundamental roles in most biological processes. Deregulation of miRNA expression is a hallmark of most cancers and further investigation of mechanisms controlling miRNA biogenesis is needed. The double stranded RNA-binding protein, NF90 has been shown to act as a competitor of Microprocessor for a limited number of primary miRNAs (pri-miRNAs). Here, we show that NF90 has a more widespread effect on pri-miRNA biogenesis than previously thought. Genome-wide approaches revealed that NF90 is associated with the stem region of 38 pri-miRNAs, in a manner that is largely exclusive of Microprocessor. Following loss of NF90, 22 NF90-bound pri-miRNAs showed increased abundance of mature miRNA products. NF90-targeted pri-miRNAs are highly stable, having a lower free energy and fewer mismatches compared to all pri-miRNAs. Mutations leading to less stable structures reduced NF90 binding while increasing pri-miRNA stability led to acquisition of NF90 association, as determined by RNA electrophoretic mobility shift assay (EMSA). NF90-bound and downregulated pri-miRNAs are embedded in introns of host genes and expression of several host genes is concomitantly reduced. These data suggest that NF90 controls the processing of a subset of highly stable, intronic miRNAs.

| S-EPMC7337520 | biostudies-literature

Targeted determination of pri-miRNA processing

Project description:Targeted sequencing at Microprocessor cleavage sites in pri-miRNAs to determine processing efficiency of several pri-miRNAs in vivo in one experiment

2020-05-01 | GSE149631 | GEO

Targeted determination of pri-miRNA processing

Project description:Targeted sequencing at Microprocessor cleavage sites in pri-miRNAs to determine processing efficiency of several pri-miRNAs in vivo in one experiment

2020-04-17 | GSE148756 | GEO

Targeted determination of pri-miRNA processing

Project description:Targeted determination of pri-miRNA processing

| PRJNA625651 | ENA

Targeted determination of pri-miRNA processing

Project description:Targeted determination of pri-miRNA processing

| PRJNA629495 | ENA

High-throughput in vitro DROSHA processing on 38,880 pri-miRNA variants

Project description:To investigate the precise processing mechanism of pri-miRNA, we prepared 38,880 pri-miRNA variants which contain unique barcode sequences (Fang and Bartel, Mol Cell, 60: 131) and performed high-throughput processing experiments.

2018-12-13 | GSE117600 | GEO

High-throughput in vitro DROSHA processing on human pri-miRNA variants

Project description:The in vitro high-throughput human pri-miRNA processing assays were conducted to check whether mismatches and wobble base pairs in the upper stem of pri-miRNAs affects the DROSHA cleavage.

2020-02-28 | GSE142140 | GEO

HASTY modulates miRNA biogenesis by linking pri-miRNA transcription and processing

Project description:HASTY modulates miRNA biogenesis by linking pri-miRNA transcription and processing

| PRJEB42556 | ENA

The high-throughput human pri-miRNA processing assays

Project description:The high-throughput human pri-miRNA processing assays

| PRJNA890620 | ENA