Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Deep sequencing of human fibroblastic cell line IMR90, either proliferating or senescent, expressing either empty vector or Parkin, with or without CCCP treatment

ABSTRACT: Deep sequencing of human fibroblastic cell line IMR90, either proliferating or senescent, expressing either empty vector or Parkin, with or without CCCP treatment

PROVIDER: PRJNA547764 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9214710_1.fastq.gz	Fastqsanger.gz
	SRR9214710_2.fastq.gz	Fastqsanger.gz
	SRR9214720_1.fastq.gz	Fastqsanger.gz
	SRR9214720_2.fastq.gz	Fastqsanger.gz
	SRR9214711_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 36

Similar Datasets

Deep sequencing of human fibroblastic cell line IMR90, either proliferating or senescent, expressing either empty vector or Parkin, with or without CCCP treatment

Project description:Gene expression changes were compared in proliferating and senescent human IMR90 cells, with either empty vector, Parkin, or Parkin+CCCP. Method: RNA was extracted, in triplicate, then sequenced with an Illumina NextSeq 500 sequencer. Sequence reads passing the quality control filters were aligned using Tophat2 and then analysed with Cufflinks.

2020-06-30 | GSE132369 | GEO

Expression data from proliferating and senescent IMR90 cells

Project description:Total RNA was isolated from proliferating and senescent IMR90 cells to compare gene-expression to the changes in nucleolus-association in proliferating and senescent IMR90 cells.

2017-06-18 | GSE80447 | GEO

Deep sequencing of human fibroblastic cell line IMR90, either proliferating or senescent, with or without TSA treatment.

Project description:Gene expression changes were compared in proliferating and senescent human IMR90 cells, with or without TSA treatment. Method: RNA was extracted, in triplicate, then sequenced with an Illumina NextSeq 500 sequencer. Sequence reads passing the quality control filters were aligned using Tophat2 and then analysed with Cufflinks.

2020-06-30 | GSE132370 | GEO

Phosphorylation level of SIRT1 in proliferating and senescent IMR90 cells

Project description:Mammalian SIRT1 is a central regulator of metabolism and aging. This project is to analyze global phosphorylation levels of mammalian SIRT1 in proliferating and senescence states using human lung fibroblast IMR90, in order to explore the post-translational regulation of SIRT1 protein upon cellular senescence and its potential roles in the regulatory mechanisms of SIRT1 homeostasis.

2020-09-29 | PXD020081 | Pride

Deep sequencing of human fibroblastic cell line IMR90, either proliferating or senescent, with or without TSA treatment.

Project description:Deep sequencing of human fibroblastic cell line IMR90, either proliferating or senescent, with or without TSA treatment.

| PRJNA547766 | ENA

Effect of mitochondria deficiency on senescence-associated gene expression

Project description:We used parkin âoverexpressing MRC5 fibroblasts to investigate the role of mitochondria deficiency on senescence-associated gene expression. RNA-seq analysis on proliferating and senescent Parkin-expressing MRC5 fibroblasts treated with CCCP (treated) or DMSO (Untreated).

2016-01-12 | E-GEOD-76125 | biostudies-arrayexpress

Expression data from PD32 and PD88 IMR90

Project description:IMR90 cells were passaged until replicative senescence and compared with proliferating cells.

2014-11-26 | GSE53356 | GEO

Expression data from PD32 and PD88 IMR90

Project description:IMR90 cells were passaged until replicative senescence and compared with proliferating cells. We used RNA-Seq to detail the global programme of gene expression in human IMR90 replicative induced senescence

2014-11-26 | E-GEOD-53356 | biostudies-arrayexpress

Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-dependent Mitophagy

Project description:Mitophagy, the selective degradation of mitochondria by autophagy, affects defective mitochondria following damage or stress. At the onset of mitophagy, parkin ubiquitylates proteins on mitochondrial outer membrane (MOM). While the role of parkin at the onset of mitophagy is well understood, less is known about its activity during later stages of the process. Here we used HeLa cells expressing catalytically active or inactive parkin to perform temporal analysis of the proteome, ubiquitylome and phosphoproteome during 18 hours after induction of mitophagy by mitochondrial uncoupler CCCP. Abundance profiles of proteins downregulated in parkin-dependent manner revealed a stepwise, “outside-in” directed degradation of mitochondrial subcompartments. While ubiquitylation of MOM proteins was enriched among early parkin-dependent targets, numerous mitochondrial inner membrane, matrix and cytosolic proteins were also found ubiquitinated at later stages of mitophagy. Phosphoproteome analysis revealed a possible cross-talk between phosphorylation and ubiquitylation during mitophagy on several key parkin targets, such as VDAC1/2.

2022-01-17 | PXD027586 | Pride

Gene expression data from IMR90 Control, IMR90 HRAS-G12V, IMR90 HRAS-G12V+shDOT1L, IMR90 DOT1L Overexpression

Project description:Gene expression data from IMR90 Control, IMR90 HRAS-G12V, IMR90 HRAS-G12V+shDOT1L, IMR90 DOT1L Overexpression

| PRJNA658579 | ENA