Project description:Deep sequencing of mRNA from the halictid Part of the Lasioglossum albipes WGS project (BioProject ID: PRJNA174755) Analysis of ploy(A)+ RNA of different specimens:whole body from the halictid (Lasioglossum albipes)
Project description:The IkB kinase (IKK) is considered to control gene expression primarily through activation of the transcription factor NF-kB. However, we show here that IKK additionally regulates gene expression on post-transcriptional level. IKK interacted with several mRNA binding proteins, including a (P)-body scaffold protein, termed Enhancer of Decapping 4 (EDC4). IKK bound to and phosphorylated EDC4 in a stimulus-sensitive manner, leading to co-recruitment of P-body components, mRNA Decapping Proteins 1a and 2 (DCP1a and DCP2) and to an increase in P-body numbers. Using RNA sequencing, we identified scores of transcripts whose stability was regulated via the IKK-EDC4 axis. Strikingly, in the absence of stimulus IKK-EDC4 promoted destabilization of pro-inflammatory cytokines and regulators of apoptosis. Our findings expand the reach of IKK beyond its canonical role as a regulator of transcription.
Project description:Whole body SIRT6 overexpression (SIRT6-tg) extends lifespan. Here we examine the effect of SIRT6 overexpression on liver mRNA profile