Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Persistent epigenetics changes due to elevated temperature during early development in mature zebrafish gonads

ABSTRACT: Persistent epigenetics changes due to elevated temperature during early development in mature zebrafish gonads

PROVIDER: PRJNA549930 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9332070_1.fastq.gz	Fastqsanger.gz
	SRR9332070_2.fastq.gz	Fastqsanger.gz
	SRR9332080_1.fastq.gz	Fastqsanger.gz
	SRR9332080_2.fastq.gz	Fastqsanger.gz
	SRR9332090_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 146

Similar Datasets

Persistent epigenetics changes due to elevated temperature during early development in mature zebrafish gonads

Project description:Sexual development is a process regulated by complex interactions of molecular events that define the final sexual phenotype. Although the genetic and transcriptomic mechanisms involved in the formation of an ovary or a testis are largely studied, the epigenetic mechanisms regulating the fate of an undifferentiated gonad remain poorly understood. Using the zebrafish model (Danio rerio), we exposed larvae of two independent families to high temperature during sex differentiation (18-32 days post fertilization), and by a targeted sequencing approach, we analyzed the methylation profiles of 18 genes related to sexual development in mature gonads two months after the exposure. Gene expression patterns were measured by qPCR in order to correlate them with the DNA methylation. Methylation profiles were family-dependent. However, general patterns of sexual dimorphism were observed in the DNA methylation levels of steroidogenic enzymes (e.g. cyp19a1a, hsd17b1 and hsd11b2), transcription factors (e.g. dmrt1 and amh) and epigenetic-related (dnmt1) genes. In testes, temperature was able to alter the methylation levels of some genes by hypermethylation (cyp19a1a, cyp11a1, amh) or hypomethylation (dmrt1), whereas in ovaries, no changes in the methylation levels were observed. Our results indicate that high temperature during sex differentiation altered methylation in the adult fish gonads and as a novelty, methylation patterns of some genes (e.g., cyp11c1, hsd11b2, dmrt3a and ar) were described here for the fish time in fish.

2022-05-20 | GSE133088 | GEO

denitrification and elevated temperature

Project description:denitrification and elevated temperature

| PRJEB55872 | ENA

Expression changes in the XY fetal gonads due to iron deficiency

Project description:Iron plays an important role in epigenome regulation by acting as a cofactor.We developed a method to enrich pre-Sertoli (Nr5a1 positive(+)) cells from mouse embryonic gonads carrying Nr5a1-hCD271 transgene. To examine mRNA expression in pre-Sertoli cells treated with the iron chelator Deferoxamine (Dfo), pre-Sertoli cells were purified and introduced into RNA seq analyses. As a result, we found that the expression of many genes, including Sry, changes due to iron deficiency.

2025-02-05 | GSE287387 | GEO

Follicular development of fetal gonads under the skin of adult mice

Project description:Adult ovarian tissues or biopsies isolated from patients prior to chemotherapy or irradiation can reconstitute ovarian functions when transplanted either in the abdomen or subcutaneously. This approach avoids invasive surgery and potential risks associated with internal procedures. We investigated whether functional ovaries could develop subcutaneously from early E12.5 fetal gonads without entering meiosis in mouse model. Unexpectedly, the subcutaneously transplanted fetal gonads failed to undergo folliculogenesis, showing meiotic deficiencies, DNA repairdefects, and elevated apoptosis. Thes meiotic defects were partly due to variations in temperature and oxygen concentration. However, in vitro culture of the gonads at 37C allowed completion of meiotic prophase I. After subcutaneous transplantation, the in vitro cultured E12.5 gonads successfully underwent folliculogenesis, restoring endocrine functions. This suggests potential for using pluripotent stem cell technologies to rejuvenate ovarioids from fetal gonad-like cells, enhancing endocrine recovery and health span.

2025-05-29 | GSE282158 | GEO

EPIGENETICS OF ALM

Project description:EPIGENETICS OF ALM

| PRJNA551390 | ENA

Comparison of expression profiles of Foxp3(+)epigenetics(-) T cells, Foxp3(-)epigenetics(+) T cells, and Foxp3(+)epigenetics(+) T cells

2012-11-05 | GSE25252 | GEO

Comparison of expression profiles of Foxp3(+)epigenetics(-) T cells, Foxp3(-)epigenetics(+) T cells, and Foxp3(+)epigenetics(+) T cells

Project description:Analysis of Foxp3(+)epigenetics(-) T cells, Foxp3(-)epigenetics(+) T cells, and Foxp3(+)epigenetics(+) T cells. Results indicate regulatory T cell (Treg) ontogenesis requires two independent processes, expression of the transcription factor Foxp3 and establishment of Treg epigenetic programs induced by T cell receptor (TCR) stimulation. GFP+CD4+ and GFP-CD4+ splenocytes were sorted from DEREG and DEREG/Scurfy mice. These cells were activated with anti-CD3/CD28 antibodies, and then transduced with Foxp3-expressing retrovirus (pGCSamIN, NGFR marker). NGFR+ T cells sorted were subjected to microarray analysis (Affymetrix, mouse genome 430 2.0 array). To normalize the experimental conditions, Tregs (GFP+ T cells from DEREG) and Tconv (GFP- T cells from DEREG) were also activated and transduced with empty vector. Two replicates each.

2012-11-05 | E-GEOD-25252 | biostudies-arrayexpress

Transcriptional Responses to Elevated Temperature in Streptococcus agalactiae

Project description:In the transition from recto-vaginal colonizing organism to invasive pathogen, Streptococcus agalactiae (Group B Streptococcus, GBS) must adapt to changes in host temperature, including elevated temperatures due to host fever. To identify genes important to the survival of GBS in response to heat stress, transcriptional profiling was performed using DNA microarray analysis, comparing GBS grown at normal temperature (37˚C) to GBS exposed to elevated temperature (42˚C).

2012-11-18 | GSE14573 | GEO

Cardiac responses to elevated seawater temperature in Atlantic salmon

Project description:Long-term exposure of Atlantic salmon to 19M-BM-0C resulted in cardiac gene and protein expression changes indicating that the unfolded protein response, vascularization, remodeling of connective tissue and altered innate immune responses were part of the cardiac acclimation or response to elevated temperature Heart tissue from Atlantic salmon (RNA poooled from N=3 fish per 3 replicate tanks) reared under normal (14 deg C) and elevated (19 deg C) temperatures were sampled after 21 (short-term) and 56 days (long-term acclimation), and gene expression changes between the elevated and the control temperature were assessed using microarray analysis.

2014-01-09 | E-GEOD-53908 | biostudies-arrayexpress

Expression changes in the XY fetal gonads due to iron deficiency

Project description:Expression changes in the XY fetal gonads due to iron deficiency

| PRJNA1212543 | ENA