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Genome-wide occupancy of FLAG-MED25 and ETV4 in PC3 cells

ABSTRACT: Genome-wide occupancy of FLAG-MED25 and ETV4 in PC3 cells

PROVIDER: PRJNA551468 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9608525.fastq.gz	Fastqsanger.gz
	SRR9608526.fastq.gz	Fastqsanger.gz
	SRR9608527.fastq.gz	Fastqsanger.gz
	SRR9608528.fastq.gz	Fastqsanger.gz
	SRR9608529.fastq.gz	Fastqsanger.gz

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Genome-wide occupancy of FLAG-MED25 and ETV4 in PC3 cells

Project description:Our in vitro binding studies support a model whereby MED25 exhibits multivalent interactions with a subset of related ETS factors, ETV1/4/5. We hypothesize that the interaction would allow for coregulation of genes by ETV1/4/5 and MED25, acting perhaps to link the ETVs to the Mediator complex. To explore this possibility, we compared the genome occupancy for FLAG-tagged MED25 and ETV4 in the prostate cancer cell line PC3, which overexpresses ETV4. We also tested for relevance of MED25 and ETV4 binding to for gene expression in PC3s. We found a high degree of overlap in the FLAG-MED25 and ETV4 ChIPs datasets consistent with our model, and also identified a subset of target genes co-dependent on Med25 and ETV4.

2019-06-28 | GSE133445 | GEO

shRNA-mediated knockdown of ETV4 and MED25 in the prostate cell line PC3 reveals set of genes potentially coregulated by MED25 and ETV4

2019-06-28 | GSE133447 | GEO

Genome-wide occupancy of FLAG-MED25 and ETV4 and expression profiling of shRNA-mediated knockdown of ETV4 and MED25 in the prostate cell line PC3

Project description:Genome-wide occupancy of FLAG-MED25 and ETV4 and expression profiling of shRNA-mediated knockdown of ETV4 and MED25 in the prostate cell line PC3

| PRJNA551467 | ENA

shRNA-mediated knockdown of ETV4 and MED25 in the prostate cell line PC3 reveals set of genes potentially coregulated by MED25 and ETV4

Project description:shRNA-mediated knockdown of ETV4 and MED25 in the prostate cell line PC3 reveals set of genes potentially coregulated by MED25 and ETV4

| PRJNA551469 | ENA

The genome-wide targeting of flag (ETV4) in A549 cells

Project description:The ETS family of oncogenic TFs is emerging as crucial mediators of tumorigenesis in solid tumors. New insights into the molecular mechanisms hijacked by these factors will pave the way for novel therapeutic strategies. We have previously shown that ETV4 is the preponderant ETS factor that is associated with tumor progression and a worse prognosis in NSCLC. To determine the genome-wide chromatin binding of ETV4, we performed chromatin immunoprecipitation (ChIP)-sequencing studies in A549-shETV4 cells transfected with flag-ETV4 using flag antibody. In total, 54389 ETV4 binding peaks representing 25708 genes were identified (FC > 2), and 43.71% peaks were enriched in the promoter regions. Our ChIP-seq data showed that ETV4 is enriched in many genes that related with the wnt signaling pathway, MAPK signaling pathway, cell cycle, and autophagy, etc.

2025-11-14 | GSE228833 | GEO

Genome-wide occupancy of FLAG-MED25 and ETV4 and expression profiling of shRNA-mediated knockdown of ETV4 and MED25 in the prostate cell line PC3

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-06-28 | GSE133448 | GEO

Gene expression changes upon knockdown or over-expression of the ETS gene ETV4 in prostate cancer cell lines

Project description:Chromosomal abnormalities that give rise to elevated expression levels of the ETS genes ETV1, ETV4, ETV5, or ERG are prevalent in prostate cancer, but the function of these transcription factors in carcinogenesis is not clear. Previous work implicates ERG, ETV1, and ETV5 as regulators of invasive growth but not transformation in cell lines. Here we show that the PC3 prostate cancer cell line provides a model system to study the over-expression of ETV4. Anchorage independent growth assays and microarray analysis indicate that high ETV4 expression is critical for the transformation phenotype of PC3 cells. However, genes up-regulated upon ETV4 over-expression were very similar to genes up-regulated by ETV1 over-expression in the RWPE-1 normal prostate cell line. Together these data indicate that the ETV4 dependent transformation phenotype observed in PC3 cells is due to the genetic background of the cell line, rather than a distinct characteristic of ETV4. Furthermore, these findings suggest that the function of ETS genes in prostate cancer may differ based on other genetic alterations in a tumor. Two sets of two color experiments. First is PC3 cells expressing one of two independent ETV4 shRNAs versus PC3 cells expressing a control shRNA (luciferase). Second is RWPE-1 cells expressing 3xFlag tagged ETV4 versus RWPE-1 cells with a control (empty) vector.

2011-03-24 | E-GEOD-21209 | biostudies-arrayexpress

The genome-wide targeting of flag (ETV4) in A549 cells

Project description:The genome-wide targeting of flag (ETV4) in A549 cells

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Genome-wide occupancy of FLAG-ERG and FLAG-EHF in RWPE1 cells

Project description:Genome-wide occupancy of FLAG-ERG and FLAG-EHF in RWPE1 cells

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Gene expression changes upon knockdown or over-expression of the ETS gene ETV4 in prostate cancer cell lines

2011-03-25 | GSE21209 | GEO