Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Influenza Vaccination Primes Human Myeloid Cell Cytokine Secretion and Natural Killer Cell Function

ABSTRACT: Influenza Vaccination Primes Human Myeloid Cell Cytokine Secretion and Natural Killer Cell Function

PROVIDER: PRJNA551581 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9610450.fastq.gz	Fastqsanger.gz
	SRR9610451.fastq.gz	Fastqsanger.gz
	SRR9610442.fastq.gz	Fastqsanger.gz
	SRR9610452.fastq.gz	Fastqsanger.gz
	SRR9610443.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Influenza Vaccination Primes Human Myeloid Cell Cytokine Secretion and Natural Killer Cell Function

Project description:51 healthy adults were vaccinated with seasonal influenza vaccine and PBMC were collected before and up to 30 days after vaccination and myloid and lymphoid (NK) cell function in response to restimulation with IL-15 alone virus was assessed.

2019-08-31 | GSE133478 | GEO

Lethal Cytokine Storm Post-mRNA Booster Vaccination

Project description:Lethal Cytokine Storm Post-mRNA Booster Vaccination

| PRJNA1008117 | ENA

Influenza Vaccination Primes Human Myeloid Cell Cytokine Secretion and NK Cell Function.

Project description:Cytokine-induced memory-like (CIML) NK cells generated in response to proinflammatory cytokines in vitro and in vivo can also be generated by vaccination, exhibiting heightened responses to cytokine stimulation months after their initial induction. Our previous study demonstrated that in vitro human NK cell responses to inactivated influenza virus were also indirectly augmented by very low doses of IL-15, which increased induction of myeloid cell-derived cytokine secretion. These findings led us to hypothesize that IL-15 stimulation could reveal a similar effect for active influenza vaccination and influence CIML NK cell effector functions. In this study, 51 healthy adults were vaccinated with seasonal influenza vaccine, and PBMC were collected before and up to 30 d after vaccination. Myeloid and lymphoid cell cytokine secretion was measured after in vitro PBMC restimulation with low-dose IL-15, alone or in combination with inactivated H3N2 virus; the associated NK cell response was assessed by flow cytometry. PBMC collected 30 d postvaccination showed heightened cytokine production in response to IL-15 compared with PBMC collected at baseline; these responses were further enhanced when IL-15 was combined with H3N2. NK cell activation in response to IL-15 alone (CD25) and H3N2 plus IL-15 (CD25 and IFN-γ) was enhanced postvaccination. We also observed proliferation of less-differentiated NK cells with downregulation of cytokine receptors as early as 3 d after vaccination, suggesting cytokine stimulation in vivo. We conclude that vaccination-induced "training" of accessory cells combines with the generation of CIML NK cells to enhance the overall NK cell response postvaccination.

| S-EPMC6739223 | biostudies-literature

Radiofrquency Ablation Combined With Cytokine-induced Killer Cells for Colorectal Cancer Liver Metastases

Project description:The purpose of this study is to determine whether combining of radiofrequency ablation (RFA) and cytokine-induced killer cells (CIK) transfusion can prolong survival of patients with colorectal cancer liver metastases (CRCLM).

| 2188141 | ecrin-mdr-crc

A controlled trail of Autologous and umbilical cord blood cytokine-induced killer cells (U-CIKs) infusion on influence of the immune function in Advanced Cancer after Radiotherapy

Project description:Interventions: A:Autologous blood cytokine-induced killer cells;B:umbilical cord blood cytokine-induced killer cells;C:Routine treatment Primary outcome(s): NK cell population;CD4/CD8 ratio;Contents of plasma immunoglobulin Study Design: Non randomized control

| 2669507 | ecrin-mdr-crc

Deconvoluting global cytokine signaling networks in natural killer cells

Project description:Deconvoluting global cytokine signaling networks in natural killer cells

| PRJNA688915 | ENA

Deconvoluting global cytokine signaling networks in natural killer cells

Project description:Deconvoluting global cytokine signaling networks in natural killer cells

| PRJNA588077 | ENA

In vivo G-CSF Treatment Activates GR-SOCS1 Axis Suppressing IFN-γ Secretion by Natural Killer Cells [CUT&TAG]

Project description:Natural killer (NK) cells are lymphocytes of the innate immune system that are involved in controlling tumors or microbial infections through the production of interferon gamma (IFN-γ). Granulocyte-colony stimulating factor (G-CSF) inhibits IFN-γ secretion by NK cells, but the mechanism underlying this effect remains unclear. Here, by comparing the multi-omics profiles of human NK cells before and after in vivo G-CSF treatment, we identified a pathway that was activated in response to G-CSF treatment, which suppressed IFN-γ secretion in NK cells. Specifically, our integrative genomic strategy revealed glucocorticoid receptor (GR) activation in NK cells that mediated the genomic response to G-CSF treatment. Activated GRs can inhibit secretion of IFN-γ by promoting interactions between suppressor of cytokine signaling 1 (SOCS1) promoter and enhancer, as well as increase the expression of SOCS1. Experiments in mice confirmed that G-CSF in vivo treatment significantly down-regulated IFN-γ secretion and up-regulated GR and SOCS1 expression in NK cells. In addition, GR blockade (RU486) significantly reversed the effects of G-CSF, demonstrating that GR up-regulates SOCS1 and inhibits the production of IFN-γ by NK cells.

2022-09-14 | GSE205824 | GEO

In vivo G-CSF Treatment Activates GR-SOCS1 Axis Suppressing IFN-γ Secretion by Natural Killer Cells [RNA-seq]

2022-09-14 | GSE205823 | GEO