Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single cell RNA sequencing CD45+ TIL samples from melanoma mouse model treated with leptin expressing vaccinia virus.

ABSTRACT: Single cell RNA sequencing CD45+ TIL samples from melanoma mouse model treated with leptin expressing vaccinia virus.

PROVIDER: PRJNA552281 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9626950.fastq.gz	Fastqsanger.gz
	SRR9626960.fastq.gz	Fastqsanger.gz
	SRR9626970.fastq.gz	Fastqsanger.gz
	SRR9626951.fastq.gz	Fastqsanger.gz
	SRR9626961.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Single cell RNA sequencing CD45+ TIL samples from melanoma mouse model treated with leptin expressing vaccinia virus.

Project description:We report the analysis of tumor infiltrating lymphocytes from a mouse melanoma model that were treated with PBS, CTRL virus or leptin expressinb vaccina oncolytic virus

2019-09-03 | GSE133699 | GEO

scRNA-seq of TIL CD45+ cells with BiAb treatment

Project description:scRNA-seq of TIL CD45+ cells with BiAb treatment

| PRJNA1119459 | ENA

Gene expression profile at single cell level of CD45+ cells from AB1 mesothelioma treated with either vaccinia virus Tiantan strain or mock-treated

Project description:To understand the immune infiltrates in the tumor microenvironment in response to vaccinia virotherapy, we used single cell RNA sequencing (scRNAseq) to analyze the diversity of total CD45+ isolated from AB1 mesothelioma

2023-12-01 | GSE221653 | GEO

CD45+ vs CD45- RCC proteome characterization

Project description:Exploration of proteome differences between CD45+ and CD45- cell types in renal cell carcinoma tumors and normal adjacent tissue patient samples.

2020-10-14 | PXD014370 | Pride

Transgenic pig over-expressing leptin as a model for female infertility

Project description:Transgenic pig over-expressing leptin as a model for female infertility

| PRJNA994072 | ENA

Orthopoxvirus vaccinia

Project description:Orthopoxvirus vaccinia Genome sequencing

| PRJNA245317 | ENA

Transgenic pig over-expressing leptin as a model for female infertility

Project description:Infertility is a growing global health concern affecting millions of couples worldwide. Both overweight and underweight individuals face reproductive issues, resulting in infertility. Due to small size and short life, mice are not necessarily suitable animal models for human infertility. While, pigs having large size, anatomical and physiological similarities with human are considered as more ideal animal model of human disease. Leptin is a well-known adipokine that serves as an endocrine signal between adiposity and fertility. However, the exact mechanisms underlying leptin's effects on reproductive function remain unclear. To shed light on this, our study focused on pigs genetically engineered to overexpress leptin. These leptin-overexpressing pigs exhibited several reproductive abnormalities, including reduced body weight and size, decreased back fat thickness, and displayed late onset of puberty, and irregular estrous behavior characterized by increased inter-estrous interval and more breeding attempts until pregnancy. This reproductive impairment in leptin pigs was followed by hormonal imbalances and altered steroidogenesis. Bulk RNA sequencing of the ovaries revealed neutrophilic infiltration followed by upregulation of inflammation related genes. To gain a deeper understanding of the cellular levels, we employed single-nucleus RNA sequencing (snRNA-seq), and found that leptin overexpression triggered immune response, suppressed follicle development and luteinization, imposing metabolism dysfunction and hormone imbalance in ovary. Further, trends of phenotypical infertility symptoms and endocrine changes in underweight female patients mirrored the alterations observed in our leptin pigs. These findings suggest that leptin-overexpressing pigs could serve as a valuable animal model for studying infertility and investigating potential therapeutic interventions.

2023-07-17 | GSE237153 | GEO

RNA-sequencing of preoptic area leptin receptor-expressing neurons from heat acclimated LepRCreHTB mice

Project description:This experiment intended to look for the transcriptome changes that happen in neuronal cells from the preoptic area of the hypothalamus expressing leptin receptors during heat acclimation conditions. 3 groups of animals were used; long-term heat-acclimated animals constantly exposed to 36˚C for 28-35 days, short-term heat-acclimated animals constantly exposed to 36˚C for 5 days, and non-habituated (non-acclimated) animals which were not exposed to heat. The animal line used was LepRCreHTB.

2024-05-06 | E-MTAB-14029 | biostudies-arrayexpress

Hyphthalamic mRNA expression of leptin receptor-expressing cell-specific Slug knockout mice

Project description:Hyphthalamic mRNA expression of leptin receptor-expressing cell-specific Slug knockout mice

| PRJNA900258 | ENA

Hyphthalamic mRNA expression of leptin receptor-expressing cell-specific Slug knockout mice

Project description:Leptin resistance is a driving force for obesity and metabolic syndrome. Slug (Snai2 or Snail2) is an epignetic factor that may play a critical role in the development of leptin resistance in obesity. We conducted a microarray to detect molecular targets of Slug in the hypothalamus.

2022-11-15 | GSE217748 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data