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RNA-sequencing (transcriptome analysis) of Desulfovibrio ferrophilus IS5 conducting electron uptake from electrodes at different potentials

ABSTRACT: RNA-sequencing (transcriptome analysis) of Desulfovibrio ferrophilus IS5 conducting electron uptake from electrodes at different potentials

PROVIDER: PRJNA553153 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9651851_1.fastq.gz	Fastqsanger.gz
	SRR9651851_2.fastq.gz	Fastqsanger.gz
	SRR9651852_1.fastq.gz	Fastqsanger.gz
	SRR9651852_2.fastq.gz	Fastqsanger.gz

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RNA-sequencing (transcriptome analysis) of Desulfovibrio ferrophilus IS5 conducting electron uptake from electrodes at different potentials

Project description:Desulfovibrio ferrophilus IS5 was incubated on indium-tin oxide (ITO) electrodes poised at −0.4 V and −0.5 V (versus standard hydrogen electrode) in H-type reactors for 10 days. Total RNA was extracted from cells after incubation, and RNA fragments were purified and transcribed into cDNA. cDNA was sequenced by NovaSeq 6000 System.

2020-08-11 | GSE133931 | GEO

Desulfovibrio ferrophilus IS5 electron uptake

Project description:Identification of putative proteins of interest that are involved in cathodic electron uptake by the novel iron-corroding strain D. ferrophilus IS5

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Desulfovibrio ferrophilus strain:IS5

Project description:Desulfovibrio ferrophilus strain IS5

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Effect of elevated ferrous ion to the gene expression of Desulfovibrio ferrophilus IS5

Project description:Microbial extracellular electron uptake (EEU) is central to bioelectrochemical processes and biocorrosion, yet its molecular mechanisms remain incompletely understood. Here, we investigate how excess Fe2+ modulates EEU in Desulfovibrio ferrophilus IS5, a strain that causes severe anaerobic iron corrosion via outer-membrane cytochromes (OMCs)-mediated electron uptake. We show that IS5 grown with elevated Fe2+ exhibits substantially enhanced EEU. This enhancement arises through two complementary mechanisms: (i) increased abundance of functional OMCs via upregulation of a cytochrome assembly protein, and (ii) an additional electron transfer route mediated by FeS nanoparticles precipitated on the IS5 outer membrane. Remarkably, IS5 with low OMCs expression but biosynthesized FeS can rapidly shift to EEU before OMCs induction. These findings suggest that during iron corrosion, when IS5 cells are embedded within thick corrosion crusts and biofilms and face both high Fe2+ concentrations and organic limitation, they exploit OMCs and FeS nanoparticles in parallel to sustain high-rate EEU from iron. This study advances the mechanistic understanding of EEU-driven iron corrosion and highlights a potential avenue for manipulating bioelectrochemical systems.

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Proteomic study of Desulfovibrio ferrophilus IS5 reveals overexpressed extracellular multi-heme cytochrome associated with severe microbiologically influenced corrosion

Project description:Microbiologically influenced corrosion (MIC) is recognized as a considerable threat to carbon steel asset integrity in the oil and gas industry. There is an immediate need for reliable and broadly applicable methods for detection and monitoring of MIC. Proteins associated with microbial metabolisms involved in MIC could serve as useful biomarkers for MIC diagnosis and monitoring. A proteomic study was conducted using a lithotrophically-grown bacteria Desulfovibrio ferrophilus strain IS5, which is known to cause severe electric MIC in seawater environments. Unique proteins, which are differentially and uniquely expressed during severe microbial corrosion by strain IS5, were identified. This includes the detection of a multi-heme cytochrome protein predicted to be involved in extracellular electron transfer in the presence of the carbon steel. Thus, we conclude that newly identified protein biomarker for MIC could be used to generate easy-to-implement immunoassays for reliable detection of microbiological corrosion in the field.

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Effect of elevated ferrous ion to the gene expression of Desulfovibrio ferrophilus IS5

Project description:Effect of elevated ferrous ion to the gene expression of Desulfovibrio ferrophilus IS5

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Project description:. In this study we show successful use of SWATH-MS for quantitative proteomic analysis of a microbial electrochemically active biofilm. Shewanella oneidensis MR-1 was grown on carbon cloth electrodes under continuous anodic electrochemical polarizations in a bioelectrochemical system. Using lactate as the electron donor, anodes serving as terminal microbial electron acceptors were operated at three different electrode potentials (+0.71V, +0.21V & -0.19V vs. SHE) and the development of catalytic activity was monitored by measuring the current traces over time. Once maximum current was reached (usually within 21-29 hours) the electrochemical systems were shut off and biofilm proteins were extracted from the electrodes for proteomic assessment.

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