Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MeRIP sequencing analysis of BJAB cell line infected with EBV

ABSTRACT: MeRIP sequencing analysis of BJAB cell line infected with EBV

PROVIDER: PRJNA553159 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9651920.fastq.gz	Fastqsanger.gz
	SRR9651921.fastq.gz	Fastqsanger.gz
	SRR9651922.fastq.gz	Fastqsanger.gz
	SRR9651923.fastq.gz	Fastqsanger.gz
	SRR9651924.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

MeRIP sequencing analysis of BJAB cell line infected with EBV

Project description:Purpose: Evaluation of the m6A modification of EBV and BJAB transcripts during EBV infection Methods: Human B lymphoma cell line BJAB was uninfected or infected with EBV for 24 hours. Total RNA from each sample were extracted. Intact mRNA was isolated from total RNA samples and then chemically fragmented to 100-nucleoside-long fragments. m6A methylated mRNAs were immunoprecipitated with anti-N6-methyadenosine (m6A) antibody (a part of the fragmented mRNAs was kept as input). Both m6A enriched mRNAs and input mRNAs were concentrated for RNA-seq library construction. Sequencing was performed using an Illumina HiSeq 4000. Results: EBV EBNA2 and BHRF1 transcripts were m6A modified and m6A modification of BJAB transcripts changed during EBV infection. Conclusions: Our study found that some EBV transcripts were m6A modified during EBV infection and EBV infection changed m6A modification profiles of BJAB transcripts.

2021-07-10 | GSE133936 | GEO

Global transcriptome analysis of BJAB cells that are modulated by EBV infection or (and) EBV BART6-3p mimics.

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are using transcriptome profiling (RNA-seq) to evaluate the effects of EBV infection or (and) EBV BART6-3p mimics on the global transcriptome of the BJAB cells. Methods: BJAB cells were transfected with negative control mimics or BART6-3p mimics for 48 h and then infected with EBV virons for 2h. RNAs were extracted by Trizol and sequenced by Solexa high-throughput sequencing service (Oebiotech, Shanghai, China). Data were extracted and normalized according to the manufacturer’s standard protocol. Results: Log-fold changes of up- or down-regulated mRNAs between the control and experiment group were selected with a significance threshold of p<0.05. There are 408 mRNAs were up-regulated and 263 were down-regulated in “EBV infection” group cells comparing to “Mock” group cells. There are 385 mRNAs were up-regulated and 246 were down-regulated in “EBV infection + Bart6-3p mimics” group cells comparing to “EBV infection + negative control mimics” group cells. Conclusions: Our study describes the global transciptome changes of BJAB cells induced by EBV infection or (and) EBV 6-3p mimics.

2018-07-01 | GSE95634 | GEO

DMS probing of and sequencing of the EBV infected BJAB-B1 transcriptome

Project description:DMS probing of and sequencing of the EBV infected BJAB-B1 transcriptome

| PRJNA865760 | ENA

DMS probing of and sequencing of the EBV infected BJAB-B1 transcriptome

Project description:To gain in cellulo, experimentally derived RNA structural information within the BJAB-B1 transcriptome

2022-08-06 | GSE210478 | GEO

MeRIP sequencing analysis of PK15 cell line infected with PRV

Project description:MeRIP sequencing analysis of PK15 cell line infected with PRV

| PRJNA863143 | ENA

Expression data from CCL185 carcinoma cell line transiently infected with EBV

Project description:Epstein-Barr virus(EBV) is associated with malignancies from lymphoid and epithelial origin. In many cases, an incomplete EBV association is noted and confoundswhate role the virus plays in oncogenesis. A number of viral proteins have been shown to interact with epigenetic factors to regulate both viral and host gene expression. Thus, we hypothesize that EBV may inadvertantly induce epigenetic alterations to the host genome that are maintained upon loss of the virus. If proven, these results would broaden EBV's role in tumorigenesis and provide a mechanism for how a tumor virus can act in a "hit-and-run" fashion. We examined EBV-induced epigenetic alterations in a setting of viral redundancy and loss. With mounting evidence that EBV can induce epigenetic alterations, we developed a transient infection model where a clonal derivative (designated cl3) from the CCL185/A549 cell line was infected with a recombinant EBV carrying neomycin resistance cassette in place of the BDLF3 open reading frame. Infected cells were passaged ten times, and then selection pressure was removed for an additional ten passages to allow for viral loss. Three transiently-infected EBV-negative clones were identified by single cell cloning (designated 10-9, 10-10, and 10-14). Uninfected parental clone and cells transfected with pcDNA3 plasmid were passaged alongside the transiently-infected clones. Each sample was analyzed in duplicate.

2012-04-10 | E-GEOD-37048 | biostudies-arrayexpress

Gene expression patterns of EBV negative BJAB B-lymphoma lines stably and conditionally expressing EBNA-1

Project description:EBNA-1 is expressed in all EBV-associated malignancies and may play a role in transcription regulation through its DNA binding ability. This is our attempt to examine the effect of EBNA-1 on the expression of cellular genes. We have compared the transcription profiles of conditional and stable EBNA-1 transfected sublines of the EBV negative B-cell lymphoma BJAB where EBNA-1 was expressed for few days, few months or years. The non-specific effects were accounted by comparing the gene expression profiles of untransfected cells or cells transfected with the empty vector or cultured in the presence or absence of doxycycline.

2010-07-17 | GSE22964 | GEO

Proteomics of EBV-infected lytic and refractory cells

Project description:Most humans are infected with Epstein-Barr virus (EBV), a cancer-causing virus. While EBV generally persists silently in B lymphocytes, periodic lytic (re-)activation of latent virus is central to its life cycle and to most EBV-related diseases. However, a substantial fraction of EBV-infected B cells and tumor cells in a population is refractory to lytic activation. This resistance to lytic activation directly and profoundly impacts viral persistence and effectiveness of oncolytic therapy for EBV+ cancers. To identify the mechanisms that underlie susceptibility to EBV-lytic activation, we used host protein-expression profiling of separated-lytic and -refractory cells.

2016-04-27 | PXD001838 | Pride

Expression in Primary Effusion Lymphoma (PEL) BCBL-1 compared to BJAB (BL cell line) and L-428 (cHD cell line)

Project description:Infection of B lymphocytes by HHV-8 is associated with the development of Primary Effusion Lymphoma (PEL). By microarray we identify differential expressions in three pro-inflammatory factors; LTA4H, TSP-1 and IL-16 in the BCBL-1 in comparison to BJAB (BL cell line) By microarray we identify differential expressions in three pro-inflammatory factors; LTA4H, TSP-1 and IL-16 in the BCBL-1 in comparison to L-428 a Classical Hodgkin’s disease cell line (cHD) as compared to BJAB a BL cell line. Keywords: Direct cell to cell comparison

2006-07-14 | GSE4464 | GEO