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Triticum aestivum cultivar ‘Chinese Spring’

ABSTRACT: Small RNA-seq from hexaploid bread wheat (Triticum aestivum) infected with wheat powdery mildew.

PROVIDER: PRJNA553193 | ENA |

REPOSITORIES: ENA

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Source:

			Action	DRS
	SRR9712610.fastq.gz	Fastqsanger.gz
	SRR9712611.fastq.gz	Fastqsanger.gz
	SRR9712612.fastq.gz	Fastqsanger.gz
	SRR9712609.fastq.gz	Fastqsanger.gz

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Triticum aestivum cultivar ‘Chinese Spring’

Project description:Transcriptomic study of powdery mildew infecting hexaploid bread wheat (Triticum aestivum).

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Project description:Mass spectrometry-based wheat proteomics is challenging because the current interpretation of mass spectrometry data relies on public databases that are not exhaustive (UniProtKB/Swiss-Prot) or contain many redundant and poor or un-annotated entries (UniProtKB/TrEMBL). Here we report the development of a manually curated database of the metabolic proteins of Triticum aestivum (hexaploid wheat), named TriMet_DB (Triticum aestivum Metabolic Proteins DataBase). The manually curated TriMet_DB was generated in FASTA format, so that it can be read directly by programs used to interpret the mass spectrometry data. Furthermore, the complete list of entries included in the TriMet_DB is reported in a freely available resource, which includes for each protein the description, the gene code, the protein family,and the allergen name (if any). To evaluate its performance, the TriMet_DB was used to interpret the mass spectrometry data acquired on the metabolic protein fraction extracted from the MEC cultivar of Triticum aestivum.

2023-03-11 | PXD037709 | Pride

mRNA-Seq for homoeologous gene expression analysis in hexaploid bread wheat

Project description:Bread wheat (Triticum aestivum) has a large, complex and hexaploid genome consisting of A, B and D homoeologous chromosome sets. Therefore each wheat gene potentially exists as a trio of A, B and D homoeoalleles, each of which may contribute differentially to wheat phenotypes. We describe a novel approach combining wheat cytogenetic resources (chromosome substitution ânullisomic-tetrasomicâ lines) with next generation deep sequencing of gene transcripts (RNA-seq), to directly and accurately identify homoeologue-specific single nucleotide variants and quantify the relative homoeoallelic contribution to gene expression. We obtained mRNA-Seq datasets from non-normalized cDNA libraries created from shoot and root tissues of the euploid bread wheat cultivar Chinese Spring, from which the nullitetra lines are derived, from complete sets of chromosome 1 and 5 nullitetras, and from extant relatives of the diploid A (Triticum urartu) and D (Aegilops tauschii) genome donors, herein referred to as A and D genome diploids

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