Project description:Semi targeted metabolic profiling from microalgal cells in acid-water methanolic extracts using UHPLC-HRMS (Orbitrap Q Exactive). Dinoflagellate Alexandrium minutum were either healthy or infected with alveolate parasite Parvilucifera infectans, and cultivated under 20 PSU or 32 PSU. Samples were unpaired and extracted on Day 14 and 21. The samples were processed using ZIC-HILIC chromatography followed by PRM aquisition in the positive polarity mode for target PSP toxins. The supplementary files include, the study and result files from Compound Discoverer, the metadata table with normalization factor, sample code and conditions. The raw files of standards used confirm retention time and MS2 fragments of samples are included as well. Comparative toxin levels of Paralytic shellfish toxin was monitored in the enodmetabolome.
