Project description:We report data obtaibed from high-throughput sequencing of small RNAs in 20 samples of follicular thyroid tumors. We analyzed a total of 4.7±1.5million reads per sample with 3 different pipelines. The main goal was to evaluate the usefulness of next generation sequencing in small RNA profiling and the concordance of its results with microarrays and qPCR. Additionally we verified published follicular thyroid tumor biomarkers in the set of our samples. Small RNA expression profiling with High Throughput Sequencing of 20 thyroid tumor samples, performed on an Illumina HiScan-SQ.
Project description:A Cartes d'Identite des Tumeurs (CIT) : Total RNAs are purified with miRNeasy kit which allows the selection of the small RNA fraction less than 100b. From these samples enriched in small RNAs, libraries are performed according to previously established protocols [Vigneault, F. et al. High-throughput multiplex sequencing of miRNA. Curr Protoc Hum Genet Chapter 11, Unit 11.12.1–10 (2012).].