Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq profiling of mouse prostate luminal cells with forced over-expression of CSF1

ABSTRACT: RNA-seq profiling of mouse prostate luminal cells with forced over-expression of CSF1

PROVIDER: PRJNA554785 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9691840.fastq.gz	Fastqsanger.gz
	SRR9691841.fastq.gz	Fastqsanger.gz
	SRR9691842.fastq.gz	Fastqsanger.gz
	SRR9691843.fastq.gz	Fastqsanger.gz
	SRR9691834.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

RNA-seq profiling of mouse prostate luminal cells with forced over-expression of CSF1

Project description:We generated a transgenic mouse harboring mouse Csf-1 transgene driven by the rat probasin promoter. Luminal cells from the 4 month old transgenic mice and WT littermate controls were FACS isolated and RNA-seq analysis was performed to compare the gene expression profiles between the two groups.

2023-01-06 | GSE134326 | GEO

ERG-driven gene expression in basal and luminal cells of mouse prostate organoids

Project description:ETS transcription factor ERG is aberrantly activated in ~50% of human prostate cancers. We found that ERG shows a preference of basal over luminal context to drive prostate cancer. However, the molecular basis of the cell context dependency remains elusive. Here we use ERG+/+;Pten-/- (EP) mouse prostate organoids with dual basal/luminal lineage reporters to profile ERG-driven gene expression programs from sorted basal vs luminal cells.

2025-04-12 | GSE294013 | GEO

Expression data from Hoxb13+ Prostate Luminal Epithelial Cells

Project description:A subpopulation of prostate luminal epithelial cells has been previously reported to be sufficient to regenerate prostatic architecture following consecutive rounds of androgen deprivation/repletion. This functional characteristic suggest prostate luminal epithelial cells as the putative cell-of-origin for castration-resistant prostate cancer - which more notizable fenotype is the lack of response to androgen deprivation thereapy. We used microarrays to profile the androgen-induced transcripts in luminal prostate epithelial cells involved in a cycle of prostate regression/regeneration in Hoxb13-rtTA|TetO-H2BGFP transgenic mice.

2021-04-06 | GSE171490 | GEO

G-CSF1

Project description:G-CSF1

| PRJNA957924 | ENA

G-CSF1

Project description:G-CSF1

| PRJNA957925 | ENA

Change in gene expression following CSF1 stimulation of mouse cancer prostate cell lines overexpressing the c-fms receptor.

Project description:To study the role of the c-fms tyrosine kinase receptor expression in prostate cancer cells, we generated a cell line model from the C2H mouse prostate cancer cell line overexpressing the c-fms receptor (N18-CFMS clone) and treated it or not with the c-fms ligand CSF1.

2022-12-09 | GSE216628 | GEO

RNAseq of untreated and CSF1-treated, CSF1+QVD-treated monocytes

Project description:Caspases, which are key effectors of apoptosis, have demonstrated non-apoptotic functions. One of these functions is the differentiation into macrophages of peripheral blood monocytes exposed to Colony-Stimulating Factor-1 (CSF1). Macrophage polarization plays an important role in the pathogenesis of diverse human diseases as cancer, leading us to explore if caspase inhibition would affect macrophage polarization. To explore the role of caspases in CSF1 differentiation, we used human monocytes sorted from buffy coats treated by cytokines. We reported that caspase inhibition delays the ex vivo differentiation of peripheral blood monocytes exposed to CSF1 and modifies the phenotype of generated macrophages, e.g. cell shape, surface markers. Moreover, by RNAseq, we observed that the macrophages generated in presence of CSF1 and QVD are different from CSF1-treated monocytes. This study confirms the importance of caspase activation in CSF1 differentiation.

2024-01-01 | E-MTAB-8088 | biostudies-arrayexpress

Gene expression profiling of mouse prostate over-expressing the Notch1 intracellular domain.

Project description:Molecular signaling that regulates differentiation, survival and proliferation of the prostate luminal epithelial cells has not been thoroughly understood. Herein, we show that increased canonical Notch1 activity suppresses terminal differentiation of prostate luminal epithelial cells but is insufficient to transform. Augmented Notch1 activity delays anoikis of luminal epithelial cells by augmenting NF-κB activity independent of Hes-1, stimulates luminal cell proliferation by potentiating the PI3K-AKT signaling, and rescues the capacities of a fraction of prostate luminal epithelial cells for unipotent differentiation in vivo and short-term self-renewal in vitro. Epithelial cell-autonomous AR signaling is dispensable for the Notch-mediated cellular survival and proliferation. This study reveals a previously unappreciated role of Notch in prostatic luminal epithelial cell differentiation, supports the presence of a lineage hierarchy within the prostate luminal epithelial cells, and implies a pro-metastatic function of Notch signaling during prostate cancer progression.

2014-08-07 | GSE53036 | GEO

Differentiated prostate luminal cells acquire enhanced regenerative potential after androgen ablation

Project description:Cells in the prostate are postulated to have stem cell properties based on organ regeneration following castration. Through single cell RNA-seq (scRNA-Seq) analysis, we identify a rare luminal cell population in the mouse prostate that expresses stem-like markers (Sca1+, Psca+), as well as a larger population of more differentiated cells (Nkx3.1+, Pbsn+, CD133/Prom1+). Unexpectedly, both populations acquire enhanced organoid regeneration potential following castration and contribute equipotently to prostatic regeneration, as revealed by lineage tracing. Regeneration is mediated, in part, by androgen-driven expression of Nrg2, Igf1, Fgf10 and Rspo3 by distinct populations of mesenchymal cells acting in a paracrine fashion on luminal cells. Human prostate tissue contains similar differentiated and stem-like luminal subpopulations which also, collectively. acquire enhanced regenerative potential after androgen ablation therapy. Thus, nearly all luminal cells that persist post-castration contribute to prostate regeneration, not just rare stem cells.

2020-05-01 | GSE146811 | GEO

Gene expression profiling of mouse prostate over-expressing the Notch1 intracellular domain.

2014-08-07 | E-GEOD-53036 | biostudies-arrayexpress