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Direct and Specific Functional Evaluation of the Nrf2 and MafG Heterodimer by Introducing a Tethered Dimer into Small Maf-Deficient Cells

ABSTRACT: Direct and Specific Functional Evaluation of the Nrf2 and MafG Heterodimer by Introducing a Tethered Dimer into Small Maf-Deficient Cells

PROVIDER: PRJNA554834 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9693320.fastq.gz	Fastqsanger.gz
	SRR9697910.fastq.gz	Fastqsanger.gz
	SRR9693301.fastq.gz	Fastqsanger.gz
	SRR9693321.fastq.gz	Fastqsanger.gz
	SRR9697911.fastq.gz	Fastqsanger.gz

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Gene expression profile in the tethered Nrf1-MafG (T-N1G) heterodimer-expressing small Maf-deficient cells [RNA-seq]

Project description:To elucidate the function of Nrf1-small Maf heterodimer, we constructed a tethered Nrf1-MafG (T-N1G) heterodimer using a flexible linker peptide and examined its function in the small Maf-deficient mouse embryonic fibroblast (MEF).

2022-01-30 | GSE188787 | GEO

Gene expression profile in the tethered Nrf2-MafG (T-N2G) heterodimer-expressing small Maf-deficient cells [RNA-seq]

Project description:To elucidate the function of Nrf2-small Maf heterodimer, we constructed a tethered Nrf2-MafG (T-N2G) heterodimer using a flexible linker peptide and examined its function in the small Maf-deficient mouse embryonic fibroblast (MEF).

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Genome wide-binding profile of tethered Nrf1-MafG (T-N1G) heterodimers in the small Maf-deficient cells [ChIP-seq]

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Genome wide-binding profile of tethered Nrf2-MafG (T-N2G) heterodimers in the small Maf-deficient cells [ChIP-seq]

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Target Gene Diversity of the Nrf1-MafG Transcription Factor Revealed by a Tethered Heterodimer

Project description:Target Gene Diversity of the Nrf1-MafG Transcription Factor Revealed by a Tethered Heterodimer

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Gene expression profile in the tethered Nrf2-MafG (T-N2G) heterodimer-expressing small Maf-deficient cells [RNA-seq]

Project description:Gene expression profile in the tethered Nrf2-MafG (T-N2G) heterodimer-expressing small Maf-deficient cells [RNA-seq]

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Gene expression profile in the tethered Nrf1-MafG (T-N1G) heterodimer-expressing small Maf-deficient cells [RNA-seq]

Project description:Gene expression profile in the tethered Nrf1-MafG (T-N1G) heterodimer-expressing small Maf-deficient cells [RNA-seq]

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Direct and Specific Functional Evaluation of the Nrf2 and MafG Heterodimer by Introducing a Tethered Dimer into Small Maf-Deficient Cells.

Project description:A group of cytoprotective genes is regulated by heterodimers composed of the cap'n'collar (CNC) family member Nrf2 and one of the small Maf (sMaf) proteins (MafF, MafG, or MafK) through the antioxidant response element (ARE, also referred to as the CNC-sMaf binding element [CsMBE]). Many lines of evidence support this model; however, a direct and specific evaluation of the Nrf2-sMaf heterodimer remains to be executed. To address this issue, we constructed a tethered Nrf2-MafG (T-N2G) heterodimer using a flexible linker peptide. We then introduced the T-N2G construct into cells lacking all three sMaf proteins to specifically evaluate the function of the tethered heterodimer without interference from other endogenous CNC-sMaf heterodimers or sMaf homodimers. In response to an Nrf2 activator, diethyl maleate, the T-N2G protein can widely activate the target genes of Nrf2 but not those of Nrf1, such as proteasome subunit genes. Genome-wide binding analysis showed that the T-N2G protein preferentially bound to the CsMBE motifs in the regulatory regions of the Nrf2 target genes. These results provide direct evidence that the Nrf2-MafG heterodimer acts as a transcriptional activator of Nrf2-dependent genes and show that this assay system will be a powerful tool to specifically examine the function of other CNC-sMaf heterodimers.

| S-EPMC6766697 | biostudies-literature

Direct and Specific Functional Evaluation of the Nrf2 and MafG Heterodimer by Introducing a Tethered Dimer into Small Maf-Deficient Cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-08-06 | GSE134353 | GEO

Comparison of gene expression profiles of MafF-/-: MafG-/-: MafK-/- embryos and MafF-/-: MafG+/+: MafK-/- embyos

Project description:MafF-/-: MafG+/+: MafK-/- mice are viable, while MafF-/-: MafG-/-: MafK-/- mice are embryonic lethal. To get an insight into the cause of the lethality of small Maf triple knockout mice, transcriptome analysis was performed using whole embyos of MafF-/-: MafG-/-: MafK-/- at E10.5 and those of MafF-/-: MafG+/+: MafK-/- at E9.5 or E10.5. Because MafF-/-: MafG-/-: MafK-/- embryos exhibit growth retardation, the gene expression profile of MafF-/-: MafG-/-: MafK-/- embryos at E10.5 was compared with that of MafF-/-: MafG+/+: MafK-/- embyos at E9.5. The gene expression profile of MafF-/-: MafG+/+: MafK-/- embryos at E10.5 was also examined as an alternative control. Total RNA was prepared from pooled three embryos for each sample.

2012-01-09 | E-GEOD-29558 | biostudies-arrayexpress

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