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Gene expression profile in porcine ovarian granulosa cells during short-term in vitro culture

ABSTRACT: Gene expression profile in porcine ovarian granulosa cells during short-term in vitro culture

PROVIDER: PRJNA554853 | ENA |

REPOSITORIES: ENA

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Gene expression profile in porcine ovarian granulosa cells during short-term in vitro culture

Project description:The porcine ovarian granulosa cells, when analysed during in vitro cultures, may provide new interesting data on the processes associated with in vitro culture of porcine oocytes, as well as assisted reproduction and in vitro fertilisation processes used in the livestock industry. Porcine ovarian granulosa cells were obtained from 43 crossbred Landrace gilts with a median age of 170 days and weight of 98 kg after slaugheter. The cells were harvested after 0h, 48h, 96h and 144h after begining of culture and submited to RNA isolation and microarray analisys. In this study, we demonstrated the gene expression profile of short term primary cultured porcine ovarian granulosa cells.

2019-07-17 | GSE134361 | GEO

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Project description:Granulosa cells (GCs) have many endocrine functions. However, in long-term in vitro culture GCs can change their properties. GCs were collected from hyper-stimulated ovarian follicles from woman during IVF procedures. They were cultured in in vitro long-term culture. RNA was collected after 1, 7, 15 and 30 days of culture. Expression microarrays were used for analysis, which allowed to identify groups of genes characteristic for particular cellular processes. In this study, we demonstrated the gene expression profile of long time primary cultured human ovarian granulosa cells.

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New Gene Markers of Angiogenesis and Blood Vessels Development in Porcine Ovarian Granulosa Cells during Short-Term Primary Culture In Vitro.

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YTHDF2 mediates the action of BDNF stimulating proliferation of porcine follicle granulosa cells

Project description:Total RNA of porcine follicle granulosa cells from different groups was extracted and RNA purity and quality control were performed.

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Gene expression profile in porcine buccal pouch mucosal cells during long-term in vitro culture

Project description:The most experiments about buccal pouch mucosal cells were investigated in mice and hamster. In these studies we have used porcine buccal pouch mucosal cells cultured primarily in vitro to investigate the expression profile of new molecular markers of mucosal wounding, vascularization and proliferation. In this study, we demonstrated the gene expression profile of long time primary cultured porcine buccal pouch mucosal cells.

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