Ontology highlight
ABSTRACT:
PROVIDER: PRJNA555403 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR9712270_1.fastq.gz | Fastqsanger.gz | |||
| SRR9712270_2.fastq.gz | Fastqsanger.gz | |||
| SRR9712280_1.fastq.gz | Fastqsanger.gz | |||
| SRR9712280_2.fastq.gz | Fastqsanger.gz | |||
| SRR9712290_1.fastq.gz | Fastqsanger.gz |
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Project description:<p>Background: Capsaicin, a natural alkaloid in chili peppers, regulates glycemic levels, however, its mechanisms and therapeutic potential remain unclear. This study aimed to elucidate the role of gut microbiota and their metabolites in mediating capsaicin’s glycemic regulatory effects. We conducted experiments in specific pathogen-free (SPF) and germ-free (GF) mice, transient receptor potential vanilloid 1 (TRPV1) receptor ablation studies and fecal microbiota transplantation (FMT) to demonstrate the involvement of gut microbiota in capsaicin-mediated glycemic control. Metagenomics and metabolomics analyses were employed to identify key microbial strains and metabolic pathways. Keystone strains and metabolites were supplemented in GF mice without capsaicin intervention to validate their effects on glycemic regulation. In vitro co-culture experiments were performed to investigate the mutualistic relationships among keystone strains under capsaicin treatment.</p><p>Results: Capsaicin ameliorates glycemic dysregulation in mice by modulating the gut microbiota, independently of TRPV1 signaling. Gut microbiota altered by capsaicin promote the production of 5-aminolevulinic acid (5-ALA), which contributes to heme synthesis and enhances glycemic control. Supplementation with Akkermansia muciniphila, Ligilactobacillus murinus or 5-ALA in GF mice recapitulates the glycemic benefits of capsaicin. Furthermore, capsaicin upregulates Akkermansia muciniphila, which in turn supports the growth of Ligilactobacillus murinus.</p><p>Conclusion: Capsaicin-induced changes in the gut microbiota promote 5-ALA synthesis, leading to improved glycemic control. These findings suggest that dietary or probiotic interventions targeting gut microbiota, particularly Akkermansia muciniphila and 5-ALA, may offer promising strategies for managing glycemic disorders, including type 2 diabetes.</p>
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