Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of transfected NC K1 cells and transfected si-NEAT1_2 K1 cells. The goals of this study are to analysis the different mRNA expression between transfected NC K1 cells and transfected si-NEAT1_2 K1 cells. Quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis. We performed mRNA-seq in the NEAT1_2 knockdown group and NC group in the K1 cell line. We found that after knockdown of NEAT1_2, 615 mRNAs were upregulated and 2364 mRNAs were downregulated.
Project description:The HGFAC gene was subjected to deletion and acquisition assays, respectively, and RNA-Seq was performed in bovine precursor adipocytes transfected with si-NC, si-HGFAC, pcDNA3.1-NC, and pcDNA3.1-HGFAC, respectively, and by comparing the groups of si-NC, pcDNA3.1-NC, si-HGFAC, and pcDNA3.1-HGFAC, respectively, the si-HGFAC, pcDNA3.1-HGFAC and pcDNA3.1-HGFAC groups were identified. HGFAC, 46 and 130 differentially expressed genes were identified, respectively. Among them, in the si-HGFAC group, 33 genes were up-regulated and 13 genes were down-regulated; in the pcDNA3.1-HGFAC group, 27 genes were up-regulated and 103 genes were down-regulated, and the screened differentially expressed genes were subsequently analyzed by GO and KEGG.
Project description:We report the high-throughput RNA sequencing on human aortic smooth muscle cells (HASMCs) transfected with siRNA targeting OTUB1 (si-OTUB1) or control siRNA (si-NC). Cells were treated with PDGF-BB for 48 hours.
Project description:In order to screen the target genes regulated by lncMGPF,we transfected the siRNA to knock down lncMGPF gene (si-lncMGPF) and negative control siRNA (si-NC), induced cells to differentiate for 2 days.
Project description:We transfected keratinocytes cell line (HaCaT cells) with si-NC and si-eIF4E, and added M5 into the cell culture medium to reveal the function of eIF4E in the in vitro psoriasis cell model.
Project description:Purpose: The purpose is to systemically identify RNA expression changes after lnc-Dpf3 knockdown in CCR7-stimulated mDCs Methods: We profiled RNA seq in mDCs transfected with si-Lnc-Dpf3 or si-NC and stimulated with or without CCR7 ligand CCL19 and CCL21. Results: CCR7 stimulation induced profound RNA expression changes in mDCs, and a set of genes were differentially expressed in lnc-Dpf3-knockdown DC
Project description:We transfected siRNA-EIF4G2 and si-NC into Hep3B cells to explore the differentially expressed genes in cells and the affected intracellular signaling pathways after EIF4G2 knockdown
