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Beauveria bassiana Mbp1 (BbMbp1) regulating genes involved in asexual development

ABSTRACT: Beauveria bassiana Mbp1 (BbMbp1) regulating genes involved in asexual development

PROVIDER: PRJNA556353 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9822280_1.fastq.gz	Fastqsanger.gz
	SRR9822280_2.fastq.gz	Fastqsanger.gz
	SRR9822281_1.fastq.gz	Fastqsanger.gz
	SRR9822281_2.fastq.gz	Fastqsanger.gz
	SRR9822282_1.fastq.gz	Fastqsanger.gz

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Beauveria bassiana Mbp1 (BbMbp1) regulating genes involved in asexual development

Project description:Gene BbMbp1 playes an essential role in asexual differentiation, a determinant to the biocontrol potential of entomopathogenic fungi. The genome-wide exprssion analysis involved in fungal development was analyzed by using high throughput sequencing (RNA-Seq). Our transcriptional profiles revealed that numerous differentially expressed genes (DEGs), of which involved in metabolism, cell transport and cell rescue, were significantly involved in asexual deveopment of B. bassiana.

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Quantitative analysis of acetylome reveals the roles of Rpd3 and Sir2 in asexual development, virulence and multiple stress responses in Beauveria bassiana.

Project description:Lysine acetylation is one of the most prevalent post-translational modifications and has since been found to regulate histone functions and affect chromatin structure and gene expression. Here, we charactered Rpd3, a histone deacetylase of Beauveria bassiana, played important roles in the deacetylation of histone H3 and H4. We unveil the role of Rpd3 in asexual development and show that a ΔRpd3 mutant strain has severe growth defects and is defective in conidiophores and blastospores development. Acetylomic analyses indicated that Rpd3 functions in asexual development and virulence and multiple stress responses via broad genetic pathways in B. bassiana. Therefore, we conclude that BbRpd3 is vital for asexual development, virulence and multiple stress responses in Beauveria bassiana.

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Quantitative analysis of proteome reveals the roles of Rpd3 and Sir2 in asexual development, virulence and multiple stress responses in Beauveria bassiana.

Project description:Lysine acetylation is one of the most prevalent post-translational modifications and has since been found to regulate histone functions and affect chromatin structure and gene expression. Here, we charactered Rpd3, a histone deacetylase of Beauveria bassiana, played important roles in the deacetylation of histone H3 and H4. We unveil the role of Rpd3 in asexual development and show that a ΔRpd3 mutant strain has severe growth defects and is defective in conidiophores and blastospores development. Proteomic analyses indicated that Rpd3 functions in asexual development and virulence and multiple stress responses via broad genetic pathways in B. bassiana. Therefore, we conclude that BbRpd3 is vital for asexual development, virulence and multiple stress responses in Beauveria bassiana.

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