Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Preliminary RNA-seq data obtained from H1975 and H661 cells exposed to AAT protein

ABSTRACT: Preliminary RNA-seq data obtained from H1975 and H661 cells exposed to AAT protein

PROVIDER: PRJNA557498 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Preliminary RNA-seq data obtained from H1975 and H661 cells exposed to AAT protein

Project description:Although the classical function of AAT is to inhibit neutrophil proteases, there is evidence that the role of AAT in inflammation is multifaceted. AAT possesses anti-inflammatory activity against immune cells, affects leukotriene B4, TNF-α, IL-8, IFN-γ and IL-1β production and expresses potent anti-apoptotic properties. In addition, AAT has been shown to inhibit the activity of natural killer cells against tumor cells, which indicates that AAT may play multiple functions during cancer development. Therefore, in a situation of occurring cancer cells higher AAT levels may favor microenvironment towards malignant progression. High expression of SERPINA1 gene encoding acute phase protein, α1-antitrypsin (AAT), is associated with poor prognosis in various tumors. Here we examined the significance of SERPINA1 and AAT protein in non-small-cell lung cancer (NSCLC) patients and NSCLC cell lines.

2019-07-31 | GSE135138 | GEO

Transcriptome profiling of Anlotinib-resistant NCI-H1975 and Anlotinib-treated Anlotinib-resistant NCI-H1975

Project description:Further to our previous study (E-MTAB-5997), here we performed transcriptome profiling on Anlotinib-resistant NCI-H1975 and Anlotinib-treated Anlotinib-resistant NCI-H1975, and would like to understand the effects of Anlotinib on Anlotinib-resistant NCI-H1975 cell, compare the different transcriptome profiling on NCI-H1975 cells and Anlotinib-resistant NCI-H1975 cells, sought to find the biomarker for explaining Anlotinib resistance.

2019-07-01 | E-MTAB-7068 | biostudies-arrayexpress

H1975 MMAB knockdown

Project description:H1975 MMAB knockdown

| PRJEB100795 | ENA

Homo sapiens strain:H1975

Project description:H1975 --0915c

| PRJNA821452 | ENA

H1975 MMAB knockdown

Project description:H1975 cells with control hairpin and two hairpins against the gene MMAB in triplicate.

2025-10-30 | E-MTAB-15737 | biostudies-arrayexpress

Erastin induced protein expression change in H1975 cells

Project description:H1975 cells were treated with DMSO and erastin, respectively. Protein was extracted and separated using SDS-PAGE electrophoresis. MS analysis was performed and protein was identified and quantified using Proteome Discoverer™ 1.3 software using the SEQUEST® search engine.

2021-09-09 | PXD020205 | Pride

Comparative proteomics of CTS effect on human lung cancer H1975

Project description:Cryptotanshinone (CTS) is a lipophilic constituent of Salvia miltiorrhiza, with a broad-spectrum anticancer activity. We have observed that CTS enhances the efficacy of gefitinib in human lung cancer H1975 cells, yet little is known about its molecular mechanism. To explore how CTS enhances H1975 cell sensitivity to gefitinib, we figured out differential proteins of H1975 cells treated by gefitinib alone or in combination with CTS using label-free liquid chromatography-mass spectrometry (LC-MS). Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI) bioinformatic analyses of the differential proteins were performed. CTS enhanced H1975 cell sensitivity to gefitinib in vitro and in vivo, with 115 and 128 differential proteins identified respectively. GO enrichment, KEGG analysis, and PPI network comprehensively demonstrated that CTS mainly impacted the redox process and fatty acid metabolism in H1975 cells. Moreover, three differential proteins were validated by RT-qPCR and Western blot. In conclusion, we used a proteomic method to study the mechanism of CTS enhancing gefitinib sensitivity in H1975 cells. Our finding reveals the potential protein targets of CTS in overcoming gefitinib resistance which may be therapeutical targets in lung cancer.

2022-02-23 | PXD031533 | Pride

Osimertinib resistant cells of H1975

Project description:Osimertinib resistant cells of H1975

| PRJNA767314 | ENA

FKBP4 knockdown effect on the gene expression of H1975 cells

Project description:H1975 cells were transfected with FKBP4 targeting shRNA and the control shRNA, then mRNA were extracted for high-throughput sequencing.

2020-09-12 | GSE157826 | GEO

Gene expression profile of cell lines 2106T, H1975 and MeWo after knockdown of PAEP.

Project description:Gene expression profile was analyzed after knockdown of PAEP in lung cancer cell lines 2106T and H1975 as well as in skin cancer cell line MeWo. The aim of the study was to investigate the role of PAEP and its protein product glycodelin in lung cancer cell lines.

2015-04-22 | GSE60888 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data