Project description:In this study, we investigated the metabolic potential of N. marina based on its complete genome sequence and performed physiological experiments to test genome-derived hypotheses. Our data confirm that N. marina benefits from additions of undefined organic carbon substrates, has adaptations to resist oxidative, osmotic and UV light-induced stress and low dissolved pCO2. Additionally, N. marina is able to grow chemoorganotrophically on formate, and is thus not an obligate chemolithoautotroph. We further investigated the metabolic response of N. marina to low (5.6 µM) O2 concentrations. In response to O2-limited conditions, the abundance of a potentially more efficient CO2-fixing pyruvate:ferredoxin oxidoreductase (POR) complex and a high-affinity cbb3-type terminal oxidase increased, suggesting a role in sustaining nitrite oxidation-driven autotrophy under O2 limitation.
Project description:Here, the complete assembly of the Moritella marina MP-1 (ATCC 15381) genome, combining Illumina and long Nanopore reads, is presented. The gapless assembly consists of a 4.7-Mb circular chromosome and a 26-kb plasmid, with a G+C content of 40.7%, and will assist in further studies of the molecular pathways in this biotechnologically significant organism.
Project description:The bacteria Cobetia marina is globally distributed in different oceanic ecosystems and is able to grow in a wide range of temperatures from 0°C to 41°C, with an optimal growth temperature close to 35°C. Its remarkable characteristics include its ability to form biofilm, produce biosurfactants, and use petroleum-derived hydrocarbons as energy and carbon source. In this context, Cobetia marina is a model for biofilm production and the production of diverse biomolecules of relevance for human and animal health, as well as for environmental protection and recovery. This dataset shows the differential expression of genes and gene regulatory circuits that explain the adaptation of Cobetia marina to different temperatures, serving as a model of evolution in response to selection pressures such as temperature changes in the marine environment. In this study, Cobetia marina was grown at 16°C, 35°C, 38°C, and 41°C to establish differential gene expression using high-throughput RNA sequencing.
Project description:This dataset contains the transcriptome sequence of Zostera marina as produced by Illumina sequencing. Four tissues were sequenced, female flower in late and early stages of development, the male flower, the root and leaf tissue.