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Transcriptional profiling of laser captured intestinal tissue from the planarian Schmidtea mediterranea

ABSTRACT: Transcriptional profiling of laser captured intestinal tissue from the planarian Schmidtea mediterranea

PROVIDER: PRJNA558547 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9899840.fastq.gz	Fastqsanger.gz
	SRR9899841.fastq.gz	Fastqsanger.gz
	SRR9899842.fastq.gz	Fastqsanger.gz
	SRR9899833.fastq.gz	Fastqsanger.gz
	SRR9899843.fastq.gz	Fastqsanger.gz

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Transcriptional profiling of laser captured intestinal tissue from the planarian Schmidtea mediterranea

Project description:Proper function and repair of the digestive system are vital to most animals. Deciphering the mechanisms involved in these processes requires an atlas of gene expression and cell types. Here, we applied laser-capture microdissection (LCM) and RNA-seq to characterize the intestinal transcriptome of Schmidtea mediterranea, a planarian flatworm that can regenerate all organs, including the gut. We identified hundreds of genes with intestinal expression undetected by previous approaches. Systematic analyses revealed extensive conservation of digestive physiology and cell types with other animals, including humans. Furthermore, spatial LCM enabled us to uncover previously unappreciated regionalization of gene expression in the planarian intestine along the medio-lateral axis, especially among intestinal goblet cells. Finally, we identified two intestine-enriched transcription factors that specifically regulate regeneration (hedgehog signaling effector gli-1) or maintenance (RREB2) of goblet cells. Altogether, this work provides resources for further investigation of mechanisms involved in gastrointestinal function, repair and regeneration.

2020-03-20 | GSE135351 | GEO

Transcriptional profiling of laser captured gonadal tissue from the planarian Schmidtea mediterranea

Project description:Transcriptional profiling of laser captured gonadal tissue from the planarian Schmidtea mediterranea

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The head-regeneration transcriptome of the planarian Schmidtea mediterranea

Project description:The head-regeneration transcriptome of the planarian Schmidtea mediterranea

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RNA sequencing of single cells from the midline posterior extremity in planarian Schmidtea mediterranea

Project description:RNA sequencing of single cells from the midline posterior tip of the tail and posterior blastema in planarian Schmidtea mediterranea identified new posterior-pole enriched genes

2019-05-25 | GSE121047 | GEO

[E-MTAB-607] Transcription profiling by high throughput sequencing of the planarian Schmidtea mediterranea during head regeneration

Project description:The head-regeneration transcriptome of the planarian Schmidtea mediterranea ArrayExpress Release Date: 2011-07-15 Publication Title: The head-regeneration transcriptome of the planarian Schmidtea mediterranea Publication Author List: Thomas Sandmann, Matthias C. Vogg, Suthira Owlarn, Michael Boutros, and Kerstin Bartscherer Person Roles: submitter Person Last Name: Sandmann Person First Name: Thomas Person Mid Initials: Person Email: t.sandmann@dkfz.de Person Phone: +49 6221 42 1954 Person Address: Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany Person Affiliation: German Cancer Research Center

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Molecular markers to characterize the hermaphroditic reproductive system of the planarian Schmidtea mediterranea

Project description:Investigation of differences in gene expression between two strains of the planarian Schmidtea mediterranea. The sexual strain are cross-fertilizing hermaphrodites with reproductive organs that develop post-embryonically and the asexual strain reproduces exclusively by transverse fission and fail to develop reproductive organs. A two chip study using total RNA recovered from asexual and sexual animals. Each chip measures the expression level of 16,797 ESTs from S. mediterranea with 10 60-mer probe pairs (PM/MM) per gene, with two-fold technical redundancy.

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Molecular markers to characterize the hermaphroditic reproductive system of the planarian Schmidtea mediterranea

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Cell type transcriptome atlas for the planarian Schmidtea mediterranea

Project description:The transcriptome of a cell dictates its unique cell-type biology. We used single-cell RNA sequencing to determine the transcriptomes for essentially every cell type of a complete animal: the regenerative planarian Schmidtea mediterranea. Planarians contain a diverse array of cell types, possess lineage progenitors for differentiated cells (including pluripotent stem cells), and constitutively express positional information, making them ideal for this undertaking. We generated data for 66,783 cells, defining transcriptomes for known and many previously unknown planarian cell types and for putative transition states between stem and differentiated cells. We also uncovered regionally expressed genes in muscle, which harbors positional information. Identifying the transcriptomes for potentially all cell types for many organisms should be readily attainable and is a powerful new approach to metazoan biology.

2018-04-17 | GSE111764 | GEO

Transcriptome analysis reveals strain-specific and conserved stemness genes in Schmidtea mediterranea

Project description:The transcriptome of the planarian Schmidtea mediterranea is not well characterized. We have used RNA-Seq to characterize the transcriptome in both sexual and asexual strains of S. mediterranea from both untreated and irradiated animals. Moreover, we have performed RNA-Seq on RNA purified from FACS sorted neoblasts and differentiated cells. Together these studies expand our understanding of the planarian transcriptome and have identified strain-specific, neoblast-specific, and conserved transcripts. RNA-Seq was performed on RNA isolated from untreated and irradiated S. mediterranea animals from both the sexual and asexual strains, on FACS purified X1 neoblasts, X2 neoblasts, and Xins differentiated cells. One of the raw data files for GSM847465 is missing. The fasta file is provided at http://genome.vcell.uchc.edu/GenomeData02/Graveley_Lab_Public_Data/Planarian/S.mediterranea_SexNIRmRNA3.fa.gz

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Transcriptome analysis reveals strain-specific and conserved stemness genes in Schmidtea mediterranea

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