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The ARID1-AGO9 complex orchestrates easiRNA movement during sperm maturation in Arabidopsis [RIP-seq]

ABSTRACT: The ARID1-AGO9 complex orchestrates easiRNA movement during sperm maturation in Arabidopsis [RIP-seq]

PROVIDER: PRJNA558575 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9904854.fastq.gz	Fastqsanger.gz
	SRR9904855.fastq.gz	Fastqsanger.gz
	SRR9904856.fastq.gz	Fastqsanger.gz
	SRR9904857.fastq.gz	Fastqsanger.gz
	SRR9904858.fastq.gz	Fastqsanger.gz

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The ARID1-AGO9 complex orchestrates easiRNA movement during sperm maturation in Arabidopsis [RNA-seq]

Project description:The ARID1-AGO9 complex orchestrates easiRNA movement during sperm maturation in Arabidopsis [RNA-seq]

| PRJNA558574 | ENA

The ARID1-AGO9 complex orchestrates easiRNA movement during sperm maturation in Arabidopsis [ChIP-seq]

Project description:The ARID1-AGO9 complex orchestrates easiRNA movement during sperm maturation in Arabidopsis [ChIP-seq]

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The ARID1-AGO9 complex orchestrates easiRNA movement during sperm maturation in Arabidopsis [small RNA-seq]

Project description:The ARID1-AGO9 complex orchestrates easiRNA movement during sperm maturation in Arabidopsis [small RNA-seq]

| PRJNA558573 | ENA

ARID1 is required to regulate and reinforce H3K9me2 in sperm cells in Arabidopsis

Project description:Heterochromatin de-condensation in companion gametic cells is conserved in both plants and animals. In plants, microspore undergoes asymmetric pollen mitosis (PMI) to produce a vegetative cell (VC) and a generative cell (GC). Subsequently, the GC undergoes pollen mitosis (PMII) to produce two sperm cells (SC). Consistent with heterochromatin de-condensation in the VC, H3K9me2, a heterochromatin mark, is barely detected in VC. However, how H3K9me2 is differentially regulated during pollen mitosis remains unclear. Here, we show that H3K9me2 is gradually evicted from the VC since PMI but remain unchanged in the GC and SC. ARID1, a pollen-specific transcription factor that facilitates PMII, promotes H3K9me2 maintenance in the GC/SC but slows down its eviction in the VC. The genomic targets of ARID1 mostly overlaps with H3K9me2 loci, and ARID1 recruits H3K9 methyltransferase SUVH6. Our results uncover that differential pattern of H3K9me2 between two cell types is regulated by ARID1 during pollen mitosis.

2024-07-24 | GSE268716 | GEO

Maintenance of H3K9me2 heterochromatin in the pollen vegetative nucleus requires ARID1 nuclear body formation

Project description:Mature pollen contains a vegetative nucleus with decondensed chromatin and two sperm cells engulfed within the vegetative cytoplasm. Chromatin de-condensation in plants usually accompanies a decrease in heterochromatin markers such as H3K9me2, H3K27me1, and H2A.W. However, H3K9 methyltransferases are detected primarily in the vegetative nucleus. This finding raises two questions: whether heterochromatin persists in the vegetative nucleus, and by what mechanism it is regulated. Here, we show that the vegetative nucleus harbors a basal level of H3K9me2, and that ARID1, an integrator coordinating pollen mitosis and heterochromatin reprogramming during sperm cell formation, promotes the maintenance of this basal level via its C-terminal tail-mediated nuclear body formation. The heterochromatin nature of ARID1 nuclear bodies is further confirmed by their colocalization with H2A.W and H3K27me1. Domain truncation analysis reveals that nuclear body formation of ARID1 depends on its C-terminal forty-nine amino acids. Deletion of this short C-terminal region abolished nuclear body formation and inhibited the interaction with H3K9 methyltransferase SUVH6. Disrupting the nuclear body localization of ARID1 significantly impairs its recruitment to H3K9me2 target loci. Therefore, the ARID1 nuclear body is necessary for maintaining H3K9me2 in the vegetative nucleus, which ultimately influences the regulation of nuclear size. Our findings demonstrate that the vegetative nucleus, despite its decondensed state, maintains a basal level of H3K9me2 that is regulated by nuclear body-localized ARID1.

2026-06-20 | GSE306636 | GEO

Cell proliferation suppressor RBR1 interacts with ARID1 to promote pollen mitosis via stabilizing DUO1 in Arabidopsis

Project description:In plants, sperm cell formation involves two rounds of pollen mitoses, in which the microspore initiates the first pollen mitosis (PMI) to produce a vegetative cell and a generative cell, then the generative cell continues the second mitosis (PMII) to produce two sperm cells. DUO1, a R2R3 Myb transcription factor, is activated in the generative cell to promote S-G2/M transition during PMII. Loss-of-function of DUO1 caused a complete arrest of PMII. Despite the importance of DUO1, how DUO1 is regulated is largely unexplored. We previously demonstrated that ARID1, an ARID transcription factor, stimulates DUO1 transcription. Here, we show that cell proliferation suppressor RBR1 interacts with ARID1 to stabilize DUO1. While the C-terminus of RBR1 is dispensable for vegetative growth, it plays a crucial role in reproductive development and facilitates interaction with ARID1. Moreover, DUO1 is a short-lived protein, ARID1 promotes the RBR1-DUO1 interaction, and RBR1 stabilizes DUO1 in a proteasome-dependent manner. Thus, RBR1 promotes DUO1-dependent PMII progression via antagonizing its repressive role in the cell cycle factors CDKA;1 and CYCB1;1. Collectively, we uncover that ARID1 and RBR1 act in concert to regulate DUO1 at both the transcriptional and post-transcriptional levels, balancing cell specification and cell division.

2025-01-02 | GSE275530 | GEO

ARID1 is required to regulate and reinforce H3K9me2 in sperm cells in Arabidopsis

Project description:ARID1 is required to regulate and reinforce H3K9me2 in sperm cells in Arabidopsis

| PRJNA1118315 | ENA

The KIF6-RBP Complex Orchestrates mRNA Transport Required for Sperm Flagellar Assembly

Project description:RNA immunoprecipitation sequencing was performed to identify transcripts associated with FMRP-containing ribonucleoprotein complexes in mouse testicular samples. Input and FMRP RIP samples were sequenced and analyzed to define RNA-binding protein-associated mRNA cargoes potentially involved in sperm flagellar assembly and function.

2026-07-15 | GSE331445 | GEO

Genome-wide maps of SWI/SNF complexes binding in WT and ARID1-less H2.35 cells

Project description:Globally, the three categories of intact SWI/SNF complexes in control cells have shared and unique binding peaks, with cBAF showing the most abundant binding sites followed by ncBAF and pBAF. In control cells, within cBAF, ARID1B bound to only a subset of ARID1A peaks. In control cells, the binding pattern of BAF45d corresponded to ARID1A patterns, but binding was virtually eliminated in ARID1-less cells. In ARID1-less cells, ARID2 binding was essentially eliminated whereas Brd9 binding was unaltered.

2020-06-10 | GSE140183 | GEO

The KIF6-RBP Complex Orchestrates mRNA Transport Required for Sperm Flagellar Assembly

Project description:The KIF6-RBP Complex Orchestrates mRNA Transport Required for Sperm Flagellar Assembly

| PRJNA1468155 | ENA