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Transcriptome analysis of brown fat in young or old mice in cold exposure

ABSTRACT: Transcriptome analysis of brown fat in young or old mice in cold exposure

PROVIDER: PRJNA558731 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9906420.fastq.gz	Fastqsanger.gz
	SRR9906430.fastq.gz	Fastqsanger.gz
	SRR9906421.fastq.gz	Fastqsanger.gz
	SRR9906412.fastq.gz	Fastqsanger.gz
	SRR9906422.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of brown fat in young or old mice in cold exposure

Project description:Purpose: We investigated the transcriptomic change in brown fat of young and old mice (wild type) through high-throughput RNA-sequencing (RNA-Seq) analysis when the mice were exposed to cold room or room temperatur. Methods: We prepared 10 of young (3 months) mice and 9 of old (24 months) mice, and kept them in cold room (4°c) or room temperature (24°c) for 24 hours. Then, we sacrified mice and extracted RNA from brown fat tissue (BAT) for RNA-seq experiment. Results: BAT of Young mice showed increased carbohydrate metabolism and glycolytic flux during cold exposure Conclusions: The thermogenesis function of BAT is accelerated on cold exposure.

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Expression Profiles of miRNAs in brown fat of mice at room temperature and cold exposure (8C)

Project description:microRNAs levels were measured in brown adipose tissue of male C57Bl6N mice that were kept at RT or during cold exposure (8Â°C) for 24 hrs. Several miRNAs including myomirs were identified to be regulated in response to cold. Brown adipose tissue from 2 groups of 8 weeks old male C57Bl6N mice at room temperature (n=3) or after cold exposure n=3) at 8Â°C for 24 hrs were excised and RNA was extracted using Trizol and the manufactureres recommended procedure. miRNA expression levels were determined using Agilent microRNA expression analysis platform.

2012-11-01 | E-GEOD-41306 | biostudies-arrayexpress

Expression Profiles of miRNAs in brown fat of mice at room temperature and cold exposure (8C)

Project description:microRNAs levels were measured in brown adipose tissue of male C57Bl6N mice that were kept at RT or during cold exposure (8°C) for 24 hrs. Several miRNAs including myomirs were identified to be regulated in response to cold.

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Short-term cold exposure induces persistent epigenomic memory in brown fat [ATAC-Seq]

Project description:Deficiency of the epigenome modulator histone deacetylase 3 (HDAC3) in brown adipose tissue (BAT) impairs the ability of mice to survive in near-freezing temperatures. Here, we report that short-term exposure to mild cold temperature (STEMCT: 15°C for 24 hours) averted lethal hypothermia of mice lacking HDAC3 in BAT (HDAC3 BAT KO) exposed to 4°C. STEMCT restored the induction of the thermogenic coactivator PGC-1a along with UCP1 at 22°C, which is greatly impaired in HDAC3-deficient BAT, and deletion of either UCP1 or PGC-1a prevented the protective effect of STEMCT. Remarkably, the protection of HDAC3 BAT KO mice from cold intolerance following STEMCT lasted for up to 7 days. Transcriptional activator C/EBPb was induced by short-term cold exposure in mouse and human BAT and, uniquely, remained high for 7 days following STEMCT. Furthermore, analysis of C/EBPb activity revealed increased binding following STEMCT at genes, including the enhancers/promotors of UCP1 and PGC-1a. These results reveal the existence of a cold-adaptive epigenomic memory mediated by C/EBPb that is persistent and HDAC3-independent.

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Short-term cold exposure induces persistent epigenomic memory in brown fat [RNA-Seq]

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Short-term cold exposure induces persistent epigenomic memory in brown fat [GRO-Seq]

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Cold-induction of Afadin in Brown Fat supports its thermogenic capacity

Project description:Transcriptomic analysis of brown fat from fat-specific Afadin depleted mice either at thermoneutrality or after 6 houts of cold exposure (5 degree celsius)

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Transcriptome profiling of brown adipose tissue during cold exposure

Project description:We applied a deep-sequencing based method – digital gene expression profiling (DGEP), to investigate gene expression in interscapular brown adipose tissue (iBAT), inguinal white adipose tissue (iWAT) and epididymal white adipose tissue (eWAT) in acute cold exposure

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Transcriptome profiling of brown adipose tissue during cold exposure

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