Next Generation Sequencing Facilitates Quantitative Analysis of HePG2i cell line with and without DOX induced GATA4 expression Transcriptomes
Ontology highlight
ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of HePG2i cell line with and without DOX induced GATA4 expression Transcriptomes
Project description:HePG2i cell line mRNA profiles of DOX induced GATA4 expression and non-DOX induced were generated by deep sequencing, in triplicate, using Illumina HiSeq 10000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks.
Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of HePG2i cell line with and without DOX induced GATA4 expression by obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA.
Project description:Difference of GATA4 and beta-catenin pulldown chromatin immunoprecipitated DNA in HePG2i cells line with and without DOX induced GATA4 expression
Project description:GATA4 facilitates its function as a transcriptional regulator by binding to promoter and enhancer regions of target genes in the glandular stomach.