Phrynocephalus vlangalii
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ABSTRACT:
PROVIDER: PRJNA560777 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Phrynocephalus vlangalii Transcriptome or Gene expression
| PRJNA718616 | ENA
Project description:Phrynocephalus vlangalii Gut Microbiome Raw sequence reads
| PRJNA850661 | ENA
Project description:Phrynocephalus vlangalii strain:PV1-6 Transcriptome or Gene expression
| PRJNA248542 | ENA
Project description:Revealing the mechanism of high altitude adaptation in poikilotherm: an intraspecific comparative transcriptomic analysis in a toad-headed lizard, Phrynocephalus vlangalii
| PRJNA305916 | ENA
Project description:Background: Hypoxia, low temperature and intensive ultraviolet are three of the top ecological factors that restrict the survival of high altitude living organisms. Previous studies mainly focused on the adaption mechanisms of endothermic organisms rather than poikilotherm at high altitudes. In this work, we aimed to identify several candidate genes that may be related to high-altitude adaptation in poikilotherm by comparing the transcriptomes of Phrynocephalus vlangalii from different elevations. Results: We performed Illumina sequencing for the two populations, Madoi and Maqu, about 80 million sequence reads were obtained, respectively. A total of 60,725 and 49,638 transcripts for each population were generated by de novo assembly. We also identified 140 genes that have undergone strong positive selection (Ka/Ks>1) and 352 protein-coding genes that may have experienced potential positive selection (1≥Ka/Ks>0.5) after comparison to the orthologous transcripts. By considering their functions, among 140 genes with Ka/Ks>1, 11 genes were most possibly related to high altitude adaptation, including six genes related to hypoxia adaption, three genes associated with cold adaption, and two ultraviolet adaption related genes. In addition, fourteen genes that were associated with high altitude adaptation among 352 genes that may experience potential positive selection. Finally, we discovered similar gene expressions between the two populations, but different on all the unigenes and candidate genes (Ka/Ks>0.5).Conclusions: We preliminary studied the high altitude adaptation mechanisms of P. vlangalii. By comparing the two populations inhabiting different altitudes, we identified a series of candidate genes that may have promoted adaption for the high-elevation distributed population of P. vlangalii. And it is notable that the expression level of all the candidate genes was also relatively higher than all the unigenes. Thus, future research is necessary to better understand the impacts of these candidate genes on high altitude adaptation. We also found the evidence for convergent evolution on some genes between endothermic and poikilothemic species, but further research of ectotherm are requisite to better understand the complicated mechanisms of high altitude adaptation.
2025-12-01 | GSE75992 | GEO
Project description:Phrynocephalus przewalskii Genome sequencing
| PRJNA560872 | ENA
Project description:Phrynocephalus forsythii Genome Data
| PRJNA699736 | ENA
Project description:Phrynocephalus versicolor | Genome sequencing
| PRJNA738137 | ENA
Project description:Extreme environmental conditions at high altitude, such as hypobaric hypoxia, low temperature, and strong UV radiation, pose a great challenge to the survival of animals. Although the mechanisms of adaptation to high-altitude environments have attracted much attention for native plateau species, the underlying metabolic regulation remains unclear. Here, we used a multi-platform metabolomic analysis to compare metabolic profiles of liver between high- and low-altitude populations of toad-headed lizards, Phrynocephalus vlangalii, from the Qinghai-Tibet Plateau. A total of 191 differential metabolites were identified, consisting of 108 up-regulated and 83 down-regulated metabolites in high-altitude lizards as compared with values for low-altitude lizards. Pathway analysis revealed that the significantly different metabolites were associated with carbohydrate metabolism, amino acid metabolism, purine metabolism, and glycerolipid metabolism. Most intermediary metabolites of glycolysis and the tricarboxylic acid cycle were not significantly altered between the two altitudes, but most free fatty acids as well as β-hydroxybutyric acid were significantly lower in the high-altitude population. This may suggest that high-altitude lizards rely more on carbohydrates as their main energy fuel rather than lipids. Higher levels of phospholipids occurred in the liver of high-altitude populations, suggesting that membrane lipids may undergo adaptive remodeling in response to low-temperature stress at high altitude. In summary, this study demonstrates that metabolic profiles differ substantially between high- and low-altitude lizard populations, and that these differential metabolites and metabolic pathways can provide new insights to reveal mechanisms of adaptation to extreme environments at high altitude.
2024-01-16 | MTBLS6900 | MetaboLights